Technical Abstract: Few reports are available in the literature on enamel formation under nutritional deficiencies. Continuously erupting rodent incisors have considerable potential to serve as a model system for amelogenesis. Thus, we performed a study to determine the effects of boron (B) deficiency on the maturing dental enamel by using this model. Male Wistar rats, 21 days old, were used throughout. They were divided in two groups, each containing 10 animals: +B (adequate; 3 mg B/kg diet), and -B (boron-deficient; 0.07 mg/kg diet). The animals were maintained on their respective diets for 14 days and then euthanized. The mandibles were resected, fixed, and processed for embedding in paraffin and/or methyl methacrylate. Oriented histological sections of the continuously erupting incisor were obtained at the level of the mesial root of the first molar allowing access to the maturation zone of the developing enamel. Dietary treatment did not affect food intake and body weight. Histomorphometric evaluation using undecalcified sections found no statistical significant differences in the enamel thickness between the groups (P>0.05). In addition, no alterations in enamel mineralization, in terms of Ca/P ratio, were observed. The results of the present study provide evidence, for the first time, that B nutritional deficiency did not elicit alterations in the maturing dental enamel.