Technical Abstract: Accurate and rapid assays for glucose are desirable for analysis of glucose and starch in food and feedstuffs. An established colorimetric glucose oxidase-peroxidase method for glucose was modified to reduce analysis time, and evaluated for factors that affected accuracy. Time required to perform the assay was reduced by approximately 40% by decreasing incubation time and removing steps that did not affect absorbance measures. Although linear regressions of absorbance and glucose concentrations of standard solutions exceeded R^2 of 0.9997, evaluation of sum of squared residuals, root mean squared error, and significance of the quadratic term indicated that the curves were quadratic in form. Mutarotatory equilibration of glucose anomers did not appear to be the issue. Evaluation of historic data suggest that the response curve is inherently quadratic. Quadratic curves predicted glucose contents of standard solutions more accurately than linear forms; overestimations at the midpoint of the curve were 0.07, 0.85, and 1.77% for quadratic and linear equations calculated from 5 standard solutions and a linear equation calculated from the 0 and most concentrated standard solution, respectively. Hydrophilic antioxidants at levels no greater than 10 micromole ascorbic acid/0.10 g of air-dried sample did not affect absorbance values.