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United States Department of Agriculture

Agricultural Research Service

Research Project: MANAGEMENT OF TEMPERATE FRUIT NUT AND SPECIALTY CROP GENETIC RESOURCES

Location: National Clonal Germplasm Repository (Corvallis, Oregon)

Title: Highly Informative Simple Sequence Repeat (SSR) Markers for Fingerprinting Hazelnut

Authors
item Gurcan, K. - OREGON STATE UNIVERSITY
item Mehlenbacher, S. - OREGON STATE UNIVERSITY
item Bassil, Nahla

Submitted to: Acta Horticulturae
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: July 30, 2008
Publication Date: N/A

Interpretive Summary: Simple sequence repeat (SSR) or microsatellite markers have many applications in breeding and genetic studies of plants, including certification of plant identity and investigations of genetic diversity, and therefore provide information for better management of germplasm collections. They are reproducible, and technically simple to use. They can also easily detect variation at the genetic level. We developed more than 150 SSR markers for hazelnut and evaluated them using a diverse set of 50 genotypes. Some hazelnut SSR markers work also in other members of the same plant family, the Betulaceae. In this study we identify two sets of a dozen markers each that are useful for fingerprinting hazelnuts.

Technical Abstract: Simple sequence repeat (SSR) or microsatellite markers have many applications in breeding and genetic studies of plants, including fingerprinting of cultivars and investigations of genetic diversity, and therefore provide information for better management of germplasm collections. They are repeatable, co-dominant, highly polymorphic, and technically simple to use. We developed more than 150 SSR markers for hazelnut (Corylus avellana) and characterized them using a diverse set of 50 accessions. Some hazelnut SSR loci amplify other genera in the Betulaceae. In this study we identify two sets of a dozen markers each that are useful for fingerprinting hazelnut accessions. Loci were chosen based on high heterozygosity, PIC values, low frequency of null alleles, coverage of the hazelnut genome, and ease of scoring. The number of alleles per locus for these 24 primers ranged from 5-16. Polymorphic information content (PIC) values ranged from 0.50 to 0.87. Most loci segregated 1:1, 1:2:1 or 1:1:1:1 in our mapping population, allowing us to assign them to a linkage group.

Last Modified: 8/2/2014
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