Technical Abstract: To monitor the effects of feed withdrawal on the prevalence of Campylobacter, market weight turkeys from six farms were examined before and after perimarketing events (feed withdrawal, transport, and holding at the slaughterhouse). Prior to transport, birds (n = 30/farm) were slaughtered on-farm and viscera (crops, duodenum, jejunum, ileum, colon, ceca, gall bladder, spleen) were removed on the premises. Within ~ 48 hr, cohorts (n = 30/farm) from the same flock were transported to a commercial abattoir, maintained in holding sheds, slaughtered, and viscera removed. No differences in the prevalence of Campylobacter spp. were evident when individual flocks were compared pre- and post- transport. However, when data for the six farms are combined Campylobacter spp. were recovered (pre- vs post- transport) at comparable rates from the duodenum (74.7% vs 74.7%), ileum (87.3% vs, 92.7%), ceca (64% vs 57%), and colon (86.7% vs 80%), but not from either the gall bladder or spleen. After feed withdrawal, transport and holding at the abattoir, there was an overall increase in Campylobacter spp. isolated from the gall bladder at the abattoir (14.7%) when compared to on-farm levels (0%, P < 0.05). When compared to on-farm levels (3%) the overall increase in Campylobacter spp. recovered from the crop at the abattoir (24%) was significant (P < 0.05), which was associated with a detectable decline in lactic acid in the emptied crop.