Technical Abstract: Phytochemical investigations often begin with a series of crude extractions consisting of a series of solvents progressing from non-polar to polar. The crude extracts are further separated using liquid/liquid chromatography techniques to enable broad separations based on liquid solubility. Finally a series of chromatographic column purifications are chosen based on the origin fraction solubilities and thin layer chromatography results. This is usually a combination of both normal and reverse phase silica gel chromatography; however, a number of different solid phases are available. Identification of isolated bioactive constituents typically involves a combination of high resolution mass spectrometry (HRMS) and nuclear magnetic resonance (NMR) spectroscopic techniques. For novel compounds, NMR techniques almost always involve both one- and two-dimensional NMR techniques.