Title: Use of microsatellite markers to determine genetic origin of domesticated striped bass (Morone saxatilis) used in a preliminary evaluation of variation in carbohydrate utilization Authors
Submitted to: Plant and Animal Genome
Publication Type: Abstract Only
Publication Acceptance Date: November 1, 2007
Publication Date: December 12, 2007
Citation: Fuller, S.A., Rawles, S.D. 2007. Use of microsatellite markers to determine genetic origin of domesticated striped bass (Morone saxatilis) used in a preliminary evaluation of variation in carbohydrate utilization [abstract]. Plant and Animal Genome XVI, January 12-16, 2008, San Diego, CA. p.269. Technical Abstract: Hybrid striped bass (HSB) production currently ranks fourth among the U.S. seafood industry. For the HSB industry to remain globally competitive, stocks of improved domesticated broodstock must be developed. The capacity of different fish to use carbohydrate varies substantially, and Morone do not use carbohydrate well. To date, there have been no studies initiated to determine correlation between carbohydrate utilization and genetic origin on striped bass (Morone saxatilis). Even-aged half-sibling fingerlings representing 66 half-sib families were produced at Pamlico Field Station (NCSU) and transported to Stuttgart National Aquaculture Research Center as phase I fingerlings. A total of 503 fingerlings were screened with ten microsatellite markers for family assignment. Based on family origin, 35 advanced juvenile striped bass (mean weight ˜ 250 g) were assigned to one of four dietary starch inclusion levels (0, 15, 25, 35% starch) and reared in replicate 600-L round tanks (3 tanks/treatment) in a flow-through freshwater (23°C) system and fed their respective diets to apparent satiation twice a day. All diets were isonitrogenous (35% digestible protein) and isolipidic (12% digestible lipid) with increasing levels of energy provided by starch. These diets were designed to span the range and exceed the amount of starch typically found in Morone commercial feeds. Fish were weighed and a blood sample was taken to determine IGF-I profiles over the course of the experiment. Preliminary growth results of this ongoing research will be discussed, as well as its potential ramifications for industry and the National Breeding Program for the Hybrid Striped Bass Industry.