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United States Department of Agriculture

Agricultural Research Service

Research Project: INVESTIGATING THE IMPACT OF STRESS ON FOODBORNE PATHOGEN COLONIZATION IN TURKEYS Title: Antibacterial effect of trans-cinnamaldehyde on Salmonella Enteritidis and Campylobacter jejuni in chickens drinking water

Authors
item Anup, K - UNIV OF CONNECTICUT
item Darre, M - UNIV OF CONNECTICUT
item Hoagland, T - UNIV OF CONNECTICUT
item Schreiber, D - UNIV OF CONNECTICUT
item Donoghue, Ann
item Donoghue, Dan - UNIV OF ARKANSAS
item Venkitanarayanan, K - UNIV OF CONNECTICUT

Submitted to: Journal of Applied Poultry Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: September 22, 2008
Publication Date: November 1, 2008
Citation: Anup, K.J., Darre, M.J., Hoagland, T.A., Schreiber, D., Donoghue, A.M., Donoghue, D.J., Venkitanarayanan, K. 2008. Antibacterial effect of Trans-cinnamaldehyde on Salmonella Enteritidis and Campylobacter jejuni in chickens drinking water. Journal of Applied Poultry Research. 17(4):490-497.

Interpretive Summary: Salmonella Enteritidis and Campylobacter jejuni are two major food-borne pathogens in the US, accounting to more than 3 million cases of human illness annually. Chickens are the natural hosts of these bacteria. Drinking water can be a potential source of Salmonella and Campylobacter, resulting in the colonization of birds. In this study, trans-cinnamaldehyde, a natural, GRAS ingredient in cinnamon oil was evaluated for its efficacy to inactivate Salmonella Enteritidis and Campylobacter in the drinking water of chickens. Well water containing various concentrations of trans-cinnamaldehyde were inoculated with a five-strain mixture of Salmonella or Campylobacter. The samples were incubated at 12.5 or 25C for 7 days, and analyzed for bacterial population on days 0, 1, 3, 5, and 7. Results indicate that trans-cinnamaldehyde is effective in killing Salmonella and Campylobacter in water, and could potentially be used to control these pathogens in chickens through the drinking water on farms.

Technical Abstract: Salmonella Enteritidis and Campylobacter jejuni are two major food-borne pathogens in the US, accounting to more than 3 million cases of human illness annually. Chickens are the natural hosts of these bacteria. Drinking water can be a potential source of S. Enteritidis and C.jejuni, resulting in the colonization of birds. In this study, trans-cinnamaldehyde (TC), a natural, GRAS ingredient in cinnamon oil was evaluated for its efficacy to inactivate S. Enteritidis and C.jejuni in the drinking water of chickens. Well water containing 0, 0.016, 0.03 and 0.06% TC were inoculated with a five-strain mixture of S. Enteritidis or C. jejuni (6 log CFU/ml). Water samples containing 1% chicken feces or feed were also included. The samples were incubated at 12.5 or 25C for 7 days, and analyzed for bacterial population on days 0, 1, 3, 5, and 7. Duplicate samples of treatments and control were included, and the study was replicated three times. Trans-cinnamaldehyde at 0.06% inactivated Salmonella completely after 24 h in water with 1% feces at both temperatures, relative to controls. In water containing 1% feed, TC (0.06%) reduced S. Enteritidis to undetectable levels after 3 days at 12.5C or 7 days at 25C. Presence of feed or feces in water reduced the antibacterial effect of TC. The effect of TC on C. jejuni was more pronounced than that on S. Enteritidis. TC at 0.06% completely inactivated the pathogen after one day of incubation at both temperatures. The presence of feces or feed did not have any effect on the antibacterial property of TC on C. jejuni. Results indicate that TC is effective in killing S. Enteritidis and C.jejuni in water, and could potentially be used to control these pathogens in chickens through the drinking water on farms.

Last Modified: 11/26/2014
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