ARS | Publication request: Expressed gene sequences from adult ovary and adult female gut genes over-expressed upon Babesia bovis infection of Rhipcephalus (Boophilus) microplus

Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Other
Publication Acceptance Date: May 12, 2008
Publication Date: January 31, 2009
Repository URL: http://ncbi.nlm.nih.gov
Citation: Saldivar, L., Guerrero, F.D., Nene, V.M., Bendele, K.G., Scoles, G.A. 2009. Expressed gene sequences from adult ovary and adult female gut genes over-expressed upon Babesia bovis infection of Rhipcephalus (Boophilus) microplus. National Center for Biotechnology Information (NCBI). Available: http://www.ncbi.nhm.nih.gov. Accession numbers FG305399-FG309475 (Gut)and FG301341-FG305398 (Ovary).

Interpretive Summary: Rhipcephalus (Boophilus) microplus ticks are vectors of Babesia bovis, the protozoan causative agent of cattle fever, a disease which is responsible for significant production losses to cattle producers in much of Africa, Central and South America and Australia. We initiated a study of differential gene expression in Rhipicephalus (Boophilus) microplus following infection by Babesia bovis in an attempt to identify tick genes which might be critical to successful pathogen infection. These critical genes might be candidates for development of anti-Babesia control technologies. We utilized a technique called subtractive cDNA library synthesis which allowed the isolation of genes preferentially activated following the adult tick's infection with Babesia bovis. We removed the gut and ovary from engorged Babesia bovis-infected fertile R. microplus females and control uninfected females and obtained 4,058 and 4,077 sequences from subtracted cDNA Babesia bovis-infected R. microplus ovary and Babesia bovis-infected R. microplus gut libraries, respectively. Thus, we have produced DNA sequence databases consisting of genes which are over-expressed in Babesia bovis-infected ovaries and guts compared to uninfected controls. The sets of sequences have been submitted and published under GenBank Accession Numbers FG305399-FG309475 (Gut) and FG301341-FG305398 (Ovary).

Technical Abstract: Boophilus ticks are vectors of Babesia bovis, the protozoan causative agent of cattle fever, a disease which is responsible for significant production losses to cattle producers in much of Africa, Central and South America and Australia. We utilized subtractive cDNA library synthesis techniques to obtain 8,135 DNA sequences from genes over-expressed in Babesis bovis-infected B. microplus adult ovary and adult female gut compared to uninfected controls. The sets of sequences have been submitted and published under GenBank Accession Numbers FG305399-FG309475 (Gut) and FG301341-FG305398 (Ovary).