ARS | Publication request: Bacterial Community Structure of a Cattle Feedlot Pen Surface

Submitted to: ASA-CSSA-SSSA Annual Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: June 25, 2008
Publication Date: October 5, 2008
Citation: Miller, D.N., Rice, W.C. 2008. Bacterial Community Structure of a Cattle Feedlot Pen Surface [abstract]. Agronomy Society of America Annual Meeting, October 5-9, 2008, Houston, Texas. 2008 CDROM. Paper No. 745-16.

Technical Abstract: Ammonia volatilization is the primary route for nitrogen loss from cattle feedlots. An additional, but poorly studied mechanism in feedlots is aerobic nitrification. The objective of this study was to characterize the spatial and temporal variation in ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) in the context of the larger bacterial community for a cattle feedlot pen in central Nebraska. Soil samples were collected over a nine-month period from three sites within a cattle feedlot pen (feed bunk, central mound, and down gradient) and at three depths, unconsolidated surface material, shallow compacted surface material (0 to 10 cm), and deep compacted surface material (10 to 20 cm). Bacterial diversity was investigated by PCR/DGGE analysis. The general bacterial community showed the strongest group separation by pen site and date, but showed poor grouping by soil depth. The AOB and NOB communities also showed similar patterns of strongest grouping by pen site and date, but showed a tendency to group by soil depth. This investigation indicates that the larger bacterial community as well as the nitrifier community was spatiotemporally dynamic and diverse.