|Nene, Vishvanath - JCVI, ROCKVILLE, MD|
Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Other
Publication Acceptance Date: June 1, 2004
Publication Date: December 1, 2005
Repository URL: http://www.ncbi.nlm.nih.gov
Citation: Nene, V.M., Guerrero, F. 2005. Expressed genes in the southern cattle tick, Rhipicephalus (Boophilus) microplus. National Center for Biotechnology Information (NCBI). Available: http://www.ncbi.nlm.nih.gov. Assession numbers CK172439-CK192860 and CV435593-CV457691. Interpretive Summary: Boophilus ticks are important vectors of pathogens that affect the global cattle population and are responsible for significant production losses to cattle producers in much of Africa, Central and South America and Australia. These ticks are controlled primarily by pesticide application, however, resistance is becoming a problem and new control technologies are needed. The availability of DNA sequence data has revolutionized approaches to the study of biological systems and individual species. Prior to this study there were less than 200 sequences of expressed genes of Rhipicephalus (Boophilus) microplus in publicly accessible databases. We sequenced 42,512 expressed transcripts of Rhipicephalus (Boophilus) microplus. This data has been submitted and published under accession numbers CK172439-CK192860 and CV435593-CV457691 in the National Center for Biotechnology Information Public Database.
Technical Abstract: We used an EST approach to study the expressed genes of Rhipicephalus (Boophilus) microplus. A normalized cDNA library was synthesized from pooled RNA purified from tick larvae which had been subjected to different treatments, including acaricide exposure, heat shock, cold shock, host odor, and infection with the protozoan causative agent of cattle fever, Babesia bovis. We have sequenced 42,512 ESTs from R. microplus. This sequence data was used to search public protein databases and a R. microplus gene index was assembled and can be viewed at http://compbio.dfci.harvard.edu/tgi/cgi-bin/tgi/gimain.pl?gudb=b_microplus. The EST sequences have been submitted and published under accession numbers CK172439-CK192860 and CV435593-CV457691 in the National Center for Biotechnology Information Public Database.