Author
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Line, John |
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Submitted to: Poultry Science Association Meeting Abstract
Publication Type: Abstract Only Publication Acceptance Date: 5/22/2008 Publication Date: 9/20/2008 Citation: Line, J.E. 2008. Effect of sponges and gas-charged incubators on recovery of Campylobacter from broiler rinses. Poultry Science Association Meeting Abstract. Vol. 87(suppl. 1) p. 122-123. Interpretive Summary: Technical Abstract: Campylobacter spp. are microaerophilic and capnophilic microorganisms. Methods to increase exposure of Campylobacter to appropriate microaerobic conditions could theoretically improve recovery of stressed cells. The porous nature of a sponge greatly increases the sample surface area exposed to microaerobic conditions when soaked in enrichment broth. We compared recovery of Campylobacter from retail chicken carcass rinses (n=30) in bags with and without added sterile sponges. The semi-permeable bags containing the samples and an equal volume of 2X Bolton Enrichment Broth were flushed and filled with Campy-gas (5% O2, 10% CO2 and 85% N2 ) and were placed in incubators under similar microaerobic or ambient atmospheric conditions. Results indicate that adding sponges to the enrichment bags had no significant effect on Campylobacter recovery. Likewise, there was no statistically significant difference in the percentage of positive samples recovered from gas-charged bags placed in microaerobic or ambient incubators. |
