Submitted to: American Peanut Council Meeting
Publication Type: Abstract Only
Publication Acceptance Date: March 11, 2008
Publication Date: March 11, 2008
Citation: Guo, B. 2008. Peanut EST Project: Gene discovery and marker development [abstract]. Proceedings of the American Peanut Council Spring Conference, March 11-12, 2008, Alexandria, Virginia. Technical Abstract: Aflatoxin contamination caused by Aspergillus fungi is a great concern in peanut production worldwide. Pre-harvest Aspergillii infection and aflatoxin contamination are usually severe in peanuts that are grown under drought stressed conditions. Genomic research can provide new tools to study plant-microbio interaction and enhance crop genetic improvement. However, genome research in peanut is far behind other crops due to the shortage of essential genome infrastructure, tools, and resources. The objectives are to develop tools and resources and provide putative genes and EST-based markers for peanut researchers and provide microarray technology for peanut community to study peanut-Aspergillus interaction and to mitigate aflatoxin contamination in peanut. We have completed sequencing the 5’ ends of total 44,064 clones from 10 cDNA libraries, resulting over 13,824 UniESTs, which will be used for production of peanut long-oligo microarray for gene expression profiling. These sequence data have been made available to the community in order to develop genomic tools and resources for deciphering the chromosomal location and biological function of genes in the peanut genome and mitigating peanut food safety issues (CD037499 to CD038843, ES702796-ES717096, ES717097-ES724546, ES751523-ES768453). Our research objectives are two folds: 1) construction of peanut 70-mer oligo microarray in collaboration with Dr. Nierman and Dr. Yu, and 2) development of markers/genes associated with the resistance. Total 13,824 ESTs have been assembled. Oligo probes were designed by comparing peanut EST sequences against A. flavus sequences, resulting in 8,402 oligos designed from 8,402 ESTs and 5,422 ESTs that no oligos were designed. The design of the microarray will contain the 8,402 peanut genes and over 12,000 A. flavus genes. The availability of this peanut-Aspergillus array will give us an edge in studying peanut-Aspergillus interaction and help us identify genes involved in both fungal invasion and crop resistance. A panel of 16 diverse peanut genotypes has been screened for genetic diversity, and two mapping populations have been developed between Tifrunner and GT-C20 and SunOlice and NC94022 for advancement to RILs (recombinant inbred lines).