IDENTIFICATION AND CHARACTERIZATION OF GENES AFFECTING COOL AND COLD WATER AQUACULTURE PRODUCTION
Location: Cool and Cold Water Aquaculture Research
Title: Isolation and characterization of microsatellite markers in the Serra Spanish mackerel, Scomberomorus brasiliensis
| Renshaw, Mark - TEXAS A&M UNIVERSITY |
| Douglas, Kory - TEXAS A&M UNIVERSITY |
| Churney, Ann Marie - TEXAS A&M UNIVERSITY |
| Gold, John - TEXAS A&M UNIVERSITY |
Submitted to: Molecular Ecology Resources
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: April 7, 2008
Publication Date: May 1, 2009
Citation: Renshaw, M., Douglas, K., Rexroad Iii, C.E., Churney, A., Gold, J. 2009. Isolation and characterization of microsatellite markers in the Serra Spanish mackerel, Scomberomorus brasiliensis. Molecular Ecology Resources. 9:830-832.
Interpretive Summary: The Serra Spanish mackerel, Scomberomorus brasiliensis, is distributed along the Caribbean and Atlantic coasts of Central and South America, ranging from Belize to Brazil, and is an important component of artisanal fisheries in the region. Microsatellites are well suited genetic markers for providing population-genetic data and assisting with the identification of stocks that cross political boundaries and encompass varying management plans. Here, we report development and characterization of 13 new genetic markers for Spanish mackerel. An additional five genetic markers isolated originally from related species were also characterized for use in studies on Spanish mackerel. The new markers developed in this study will prove useful for future population-genetic research in this species.
Thirteen nuclear-encoded microsatellites from a genomic DNA library of Serra Spanish mackerel, Scomberomorus brasiliensis, were isolated and characterized. The microsatellites include 10 perfect repeats (8 tetranucleotide and 2 dinucleotide) and 3 imperfect repeats (2 tetranucleotide and 1 dinucleotide). An additional five microsatellites, isolated originally from two congeneric species (S. cavalla and S. niphonius), were characterized in S. brasiliensis. The number of alleles among a sample of 41 fish ranged from 3 to 35; gene diversity ranged from 0.118 to 0.978. Serra Spanish mackerel support artisanal fisheries along the Caribbean and Atlantic coasts of Central and South America, from Belize to Brazil. The microsatellites developed in the present study will be useful for studying the population-genetics of S. brasiliensis (e.g., stock structure, effective population size) and providing valuable information for managers of the fisheries.