Technical Abstract: Previously reported protocols for culture of sunshine bass larvae to fingerling size in tanks involved an initial feeding of rotifers for several days before the larvae were weaned to Artemia nauplii and prepared feed. Maintaining rotifer cultures requires space, time, equipment, supplies, and trained culturists. The rotifer cultures are often unstable, which increases risk of poor sunshine bass fingerling production in tanks. Elimination of the use of rotifers would greatly enhance the feasibility of reliable tank culture of fingerlings and should reduce production cost. This experiment compared of three treatments with three replicates per treatment. In one treatment larvae were initially fed rotifers (Brachionus plicatilis) and then weaned to Artemia nauplii (0.48 mm X 0.19 mm). In a second treatment larvae were fed Artemia nauplii throughout the experiment. In a third treatment larvae were fed microcyst Artemia nauplii (0.43 mm X 0.18 mm) for the entire experiment. Sunshine bass larvae, 4 dph, were stocked into 100-L tanks at 75 larvae/L. Larvae were fed according to the three treatments until 14 dph. Only 4.3% of the larvae fed Artemia nauplii survived. Survival was significantly higher for larvae fed microcyst Artemia nauplii and larvae fed rotifers and Artemia nauplii (37.9% and 93.6%, respectively). Larvae fed microcyst Artemia nauplii (7.26 mm SL) and larvae fed rotifers and Artemia nauplii (7.13 mm SL) were significantly longer than larvae fed Artemia nauplii (6.86 mm SL). This experiment is the first time that sunshine bass have been cultured to 14 dph on Artemia nauplii without rotifers at first feeding.