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United States Department of Agriculture

Agricultural Research Service

Research Project: MOLECULAR CHARACTERIZATION AND GASTROINTESTINAL TRACT ECOLOGY OF COMMENSAL HUMAN FOOD-BORNE BACTERIAL PATHOGENS IN THE CHICKEN Title: Quantitative techniques for the measurement and analysis of biofouling on stainless steel

Author
item Arnold, Judy

Submitted to: Society for General Microbiology
Publication Type: Abstract Only
Publication Acceptance Date: February 12, 2008
Publication Date: April 1, 2008
Citation: Arnold, J.W. 2008. Quantitative techniques for the measurement and analysis of biofouling on stainless steel. Society for General Microbiology. P 66.

Technical Abstract: A model of wet-processing conditions tested the effects of corrosive treatment on bacterial attachment to stainless steel with different surface finishes. Electropolished samples resisted surface oxidation, corresponding with the visual observation of lower discoloration than the other samples. The Z range measured by atomic force microscopy (AFM)of the electropolished sample illustrated reduced surface roughness. A tremendous amount of data can be generated for each sample. The AFM has been shown to be a useful tool for predicting bacterial attachment to surfaces. The surface roughness described the finer irregularities of surface relative to the center plane. Relative differences in the surface morphology of stainless steel finishes, including fractal dimensions, Z ranges, roughness, and other measurements corresponded by treatment with the differences in reduction of bacterial counts. Biofilm was detected by epifluorescence microscopy and quantified with image analysis software. The multiple imaging techniques showed that stainless steel surfaces can be engineered to reduce bacterial contamination, biofilm formation, and corrosion during processing under dynamic flow conditions. Therefore, the results indicated that electropolishing is the most appropriate finish for industrial applications needing to improve these parameters.

Last Modified: 11/23/2014
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