Title: Isolation, purification, and characterization of Phakopsora pachyrhizi isolates Authors
|Paul, Chandra - UNIV. OF ILLINOIS|
|Steinlage, Todd - UNIV. OF ILLINOIS|
Submitted to: APSnet Resource Center
Publication Type: Abstract Only
Publication Acceptance Date: October 30, 2007
Publication Date: December 12, 2007
Citation: Smith, D.A., Paul, C., Steinlage, T., Miles, M.R., Hartman, G.L. 2007. Isolation, purification, and characterization of Phakopsora pachyrhizi isolates [abstract]. APSnet Resource Center. 2007 National Soybean Rust Symposium, December 12-14, 2007. Louisville, KY. Available: http://www.apsnet.org/online/SBR. Technical Abstract: Soybean rust, caused by Phakopsora pachyrhizi, was first reported in the continental United States in November 2004, and is one of the most important foliar diseases of soybean worldwide. P. pachyrhizi isolates have been obtained from 2006 and 2007 across the U. S. and are being purified and maintained as either single-spore or bulk isolates. A preliminary set of soybean accessions with characterized genes for resistance have been used to evaluate pathogenic differences among isolates. Depending on the soybean line, some isolates cause an immune or hypersensitive reaction, a reddish-brown resistant reaction, or a susceptible tan reaction. Differences in reaction patterns of the isolates indicated variation in virulence. Further isolation, purification, and testing of isolates on a differential set along with DNA typing will define P. pachyrhizi variability across the U. S.