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ARS Home » Northeast Area » Boston, Massachusetts » Jean Mayer Human Nutrition Research Center On Aging » Research » Publications at this Location » Publication #218768
Title: Molecular and cellular effects of a-, and g-tocopheryl phosphate on THP-1 monocytes

Author
item ZINGG, JEAN-MARC - JM USDA HNRCA @ TUFTS
item NEGIS, YESIM - JM USDA HNRCA @ TUFTS
item GIANELLO, ROBERT - MONASH UNIV AUSTRALIA
item LIBINAKI, ROKSAN - MONASH UNIV AUSTRALIA
item OGRU, ESRA - MONASH UNIV AUSTRALIA
item Meydani, Mohsen
item AZZI, ANGELO - JM USDA HNRCA @ TUFTS

Submitted to: Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: 12/1/2007
Publication Date: 4/5/2008
Citation: Zingg, J., Negis, Y., Gianello, R., Libinaki, R., Ogru, E., Meydani, M., Azzi, A. 2008. Molecular and cellular effects of a-, and g-tocopheryl phosphate on THP-1 monocytes. Experimental Biology Annual Meeting. Abstract No. 1103.7.

Interpretive Summary:

Technical Abstract: The vitamin E derivative, alpha-tocopheryl phosphate (aTP), can be isolated from food and animal tissues in amounts of nmol/g of extracted material. Whereas the phosphate ester increases the chemical stability of alpha-tocopherol (aT), it suppresses its function as a direct free radical scavenger. Nevertheless, in cells aTP influences signal transduction and gene expression, possibly by interacting with specific enzyme(s) and/or receptor(s). Small amounts of aTP formed from aT in cultured cells further suggest a physiological role for this compound. We have now compared the inhibitory effects of aTP (acid and sodium salt) and g-tocopheryl phosphate (gTP) (acid and sodium salt) on the proliferation of THP-1 monocytic leukemia cells. aT does not affect the cell proliferation rate in these cells, whereas aTP and more potently gTP inhibit THP-1 cell proliferation. We found that one or more cell anion transporters are modulating the response of THP-1 cells to the treatment with aTP and gTP, as determined by using specific transport inhibitors, such as glybenclamide/glyburide. In addition, the effects of these vitamin E derivatives on gene expression are assessed, in particular on the inhibition of CD36 scavenger receptor gene expression.