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United States Department of Agriculture

Agricultural Research Service

Research Project: IMMUNOLOGIC AND PHARMACOLOGICAL INTERVENTIONS OF VECTOR-BORNE BABESIOSIS

Location: Animal Diseases Research

Title: Genome Sequence of Babesia bovis and Camparative Analysis of Apicomplexan Hemoprotozoa

Authors
item Brayton, Kelly - WSU
item Lau, Audrey - WSU
item HERNDON, DAVID
item Hannick, Linda - INST FOR GENOMIC RSRCH MD
item KAPPMEYER, LOWELL
item Berens, Shawn - WSU
item Bidwell, Shelby - INST FOR GENOMIC RSRCH MD
item Brown, Wendy - WSU
item Crabtree, Jonathan - INST FOR GENOMIC RSRCH MD
item Fadrosh, Doug - INST FOR GENOMIC RSRCH MD
item Feldblum, Tamara - INST FOR GENOMIC RSRCH MD
item Forberger, Heather - INST FOR GENOMIC RSRCH MD
item Haas, Brian - INST FOR GENOMIC RSRCH MD
item Freeman, Jeanne
item Khouri, Hoda - INST FOR GENOMIC RSRCH MD
item Koo, Hean - INST FOR GENOMIC RSRCH MD
item Mann, David - IMPERIAL COLLEGE, LONDON
item Norimine, Junzo - WSU
item Paulsen, Ian - INST FOR GENOMIC RSRCH MD
item Radune, Diana - INST FOR GENOMIC RSRCH MD
item Ren, Qinghu - INST FOR GENOMIC RSRCH MD
item Smith, Roger - INST FOR GENOMIC RSRCH MD
item SUAREZ, CARLOS
item White, Owen - INST FOR GENOMIC RSRCH MD
item Wortman, Jennifer - INST FOR GENOMIC RSRCH MD
item KNOWLES, DONALD
item Mcelwain, Terry - WSU
item Nene, Vishvanath - INST FOR GENOMIC RSRCH MD

Submitted to: PLoS Pathogens
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: August 30, 2007
Publication Date: October 19, 2007
Citation: Brayton, K., Lau, A.O., Herndon, D.R., Hannick, L., Kappmeyer, L.S., Berens, S.J., Bidwell, S.L., Brown, W.C., Crabtree, J., Fadrosh, D., Feldblum, T., Forberger, H.A., Haas, B.J., Howell, J.M., Khouri, H., Koo, H., Mann, D.J., Norimine, J., Paulsen, I.T., Radune, D., Ren, Q., Smith, R.K., Suarez, C.E., White, O., Wortman, J.R., Knowles Jr, D.P., Mcelwain, T.F., Nene, V.M. 2007. Genome Sequence of Babesia bovis and Camparative Analysis of Apicomplexan Hemoprotozoa. PLoS Pathogens. 3(10):1401-1413.

Interpretive Summary: The future of vaccine development for infectious diseases of domestic animals, such as those causes by complex parasites (protozoan) such as Babesia bovis require detailed information about pathogen genetics. This manuscript reports the completed annotated sequence of Babesia bovis. Babesia bovis causes disease effecting red blood cells of cattle.

Technical Abstract: Babesia bovis is an apicomplexan tick-transmitted pathogen of cattle imposing a global risk and severe constraints to livestock health and economic development. The complete genome sequence was undertaken to facilitate vaccine antigen discovery, and to allow for comparative analysis with the related apicomplexan hemoprotozoa Theileria parva and Plasmodium falciparum. At 8.2 Mbp, the B. bovis genome is similar in size to that of Theileria spp. Structural features of the B. bovis and T. parva genomes are remarkably similar, and extensive synteny is present despite several chromosomal rearrangements. In contrast, B. bovis and P. falciparum, which have similar clinical and pathological features, have major differences in genome size, chromosome number, and gene complement. Chromosomal synteny with P. falciparum is limited to microregions. The B. bovis genome sequence has allowed wide scale analyses of the polymorphic variant erythrocyte surface antigen protein (ves1 gene) family that, similar to the P. falciparum var genes, is postulated to play a role in cytoadhesion, sequestration, and immune evasion. The ;150 ves1 genes are found in clusters that are distributed throughout each chromosome, with an increased concentration adjacent to a physical gap on chromosome 1 that contains multiple ves1-like sequences. ves1 clusters are frequently linked to a novel family of variant genes termed smorfs that may themselves contribute to immune evasion, may play a role in variant erythrocyte surface antigen protein biology, or both. Initial expression analysis of ves1 and smorf genes indicates coincident transcription of multiple variants. B. bovis displays a limited metabolic potential, with numerous missing pathways, including two pathways previously described for the P. falciparum apicoplast. This reduced metabolic potential is reflected in the B. bovis apicoplast, which appears to have fewer nuclear genes targeted to it than other apicoplast containing organisms. Finally, comparative analyses have identified several novel vaccine candidates including a positional homolog of p67 and SPAG-1, Theileria sporozoite antigens targeted for vaccine development. The genome sequence provides a greater understanding of B. bovis metabolism and potential avenues for drug therapies and vaccine development.

Last Modified: 9/10/2014
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