Technical Abstract: HPLC (High-performance liquid chromatography) is the most comon technique for identifying and measuring vitamin E concentrations. A variety of good HPLC methods are available for vitamin E analysis. Reliable and sensitive methods have been developed using reversed-phased and normal-phase HPLC columns, isocratic and gradient elutions, and fluorescent, electrochemical and ultraviolet detectors. Satisfactory internal standards include tocol, tocopheryl acetate and retinyl acetate. Current methods development focuses on analysing vitamin E in difficult sample matrices, analysing vitamin E metabolites and most commonly on combining vitamin E analysis with the analysis of other micronutrients such as vitamin A and the carotenoids.