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United States Department of Agriculture

Agricultural Research Service

Research Project: DEVELOPMENT OF COLD STORAGE TECHNOLOGY FOR MASS-REARED AND LABORATORY-COLONIZED INSECTS Title: Cold storage of the adult stage of Gonatocerus ashmeadi girault: the impact on maternal and progeny quality

Authors
item Leopold, Roger
item Chen, Wen-Long - ND STATE UNIV, FARGO

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: November 1, 2007
Publication Date: November 20, 2007
Citation: Leopold, R.A., Chen, W. 2007. Cold storage of the adult stage of Gonatocerus ashmeadi girault: the impact on maternal and progeny quality [abstract]. 2007 Pierce's Disease Research Symposium. p. 42.

Technical Abstract: The effect of storage of adult G. ashmeadi at 2, 5 and 10°C on maternal and the unstored progeny fitness qualities was examined. The maternal generation did not survive 5 d exposure to 2°C and those stored at 10°C survived longer than those held at 5°C. Oviposition of the maternal generation continued for 13 d after storage at 10°C for 10 or 20 d and for 8 d for the wasps stored for 30, 40 or 50 d. After storage for 60 d, the parasitoids did not oviposit for the first 2 d and then there was only a 4 d oviposition period. Cold storage reduced the fecundity of the parents and their F1 progeny, but not the F2 generation. After 20 d storage, the fecundity of the maternal generation decreased by 47% and for 60 d storage, it was reduced 90%. The longevity of F1 parasitoids was also less than that of F2 parasitoids. After 30 d storage of the parents, the longevity of the F1 generation was reduced 49% as compared to a 10 d parental storage period. The F1 progeny of the stored female parasitoids developed about 1 d more slowly than that of the F2 and F3 generations. Moreover, cold storage caused a reduction in the incidence of parasitism by parental generation, but this effect did not extend to F1 and F2 progeny. Emergence of F1 parasitoids decreased as the length of storage of their parents increased. Further, the parental generation deposited more haploid than diploid eggs after storage for 20 d and after 50 d, production of males was increased by 132%. The sex ratios of the F2 and F3 generations did not vary with the storage duration of the ancestral generation.

Last Modified: 12/21/2014
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