Page Banner

United States Department of Agriculture

Agricultural Research Service

Title: Construction of a Fosmid Library of Cucumber (Cucumis sativus) and Comparative Analyses of the eIF4E and eIF(iso)4E Regions from Cucumber and Melon (Cucumis melo)

Authors
item Meyer, J - UW MADISON
item Deleu, W - IRTA CABRILS SPAIN
item Garcia-Mas, J - IRTA CABRILS SPAIN
item Havey, Michael

Submitted to: Molecular Genetics and Genomics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 21, 2008
Publication Date: February 14, 2008
Citation: Meyer, J.D., Deleu, W., Garcia-Mas, J., Havey, M.J. 2008. Construction of a Fosmid Library of Cucumber (Cucumis sativus) and Comparative Analyses of the eIF4E and eIF(iso)4E Regions from Cucumber and Melon (Cucumis melo). Molecular Genetics and Genomics. 279(5):473-480.

Interpretive Summary: Cucumber and melon are the most economically important and distantly related plants in the family Cucurbitaceae. We undertook comparative sequence analyses to assess similarities and orientations among genes in the cucumber and melon DNAs. A fosmid library of cucumber was synthesized as an unrestricted resource for researchers. End sequencing of random fosmids produced over 721 kilobases of cucumber DNA sequence, of which 25% was similar to ribosomal DNAs, 25% to satellite sequences, 20% to coding regions in other plants, 4% to transposable elements, 13% to mitochondrial and chloroplast sequences, and 13% showed no hits to the databases. The relatively high frequencies of ribosomal and satellite DNAs are consistent with previous analyses of cucumber DNA. Cucumber fosmids were selected and sequenced that carried eukaryotic initiation factors (eIF) 4E and iso(4E), genes associated with recessively inherited virus resistances in a number of plants. Differences in the DNA near eIF4E and eIF(iso)4E mapped independently of the zym, a recessive gene in cucumber conditioning resistance to Zucchini yellow mosaic virus, establishing that these candidate genes are not zym. Cucumber sequences were compared with melon DNA regions carrying eIF4E and eIF(iso)4E and revealed extensive sequence conservation and orientations of genes in cucumber and melon across these two independent genomic regions. These similarities are important for cucurbit geneticists and breeders to aid in the cloning of genes from both species, as well as allow for genomic resources developed for one species to be used for analyses in other species.

Technical Abstract: Cucumber and melon are the most economically important plants in the family Cucurbitaceae and are members of distinct subgenera in the genus Cucumis. We undertook comparative sequence analyses to assess synteny between the cucumber and melon genomes. A fosmid library of cucumber was synthesized as an unrestricted resource for researchers. End sequencing of random fosmids produced over 721 kilobases of cucumber genomic sequence, of which 25% was similar to ribosomal DNAs, 25% to satellite sequences, 20% to coding regions in other plants, 4% to transposable elements, 13% to mitochondrial and chloroplast sequences, and 13% showed no hits to the databases. The relatively high frequencies of ribosomal and satellite DNAs are consistent with previous analyses of cucumber DNA. Cucumber fosmids were selected and sequenced that carried eukaryotic initiation factors (eIF) 4E and iso(4E), genes associated with recessively inherited resistances to potyviruses in a number of plants. Indels near eIF4E and eIF(iso)4E mapped independently of the zym, a recessive locus conditioning resistance to Zucchini yellow mosaic virus, establishing that these candidate genes are not zym. Cucumber sequences were compared with melon BACs carrying eIF4E and eIF(iso)4E and revealed extensive sequence conservation and synteny between cucumber and melon across these two independent genomic regions. This microsynteny will aid in the cloning of orthologous genes from both species, as well as allow for genomic resources developed for one Cucumis species to be used for analyses in other species.

Last Modified: 12/18/2014
Footer Content Back to Top of Page