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United States Department of Agriculture

Agricultural Research Service

Research Project: EPIDEMIOLOGY AND MANAGEMENT OF XYLELLA FASTIDIOSA (XF) AND OTHER EXOTIC AND INVASIVE DISEASES AND INSECT PESTS Title: Microarray Analysis of Global Gene Expression of V. vinifera in Response to Xylella fastidiosa Infection

Authors
item Lin, Hong
item Harshavardhan,, Doddapaneni - UC DAVIS
item Walker,, Andrew - UC DAVIS

Submitted to: CDFA Pierce's Disease Control Program Research Symposium
Publication Type: Proceedings
Publication Acceptance Date: October 9, 2007
Publication Date: December 12, 2007
Citation: Lin, H., Harshavardhan,, D., Walker,, A.M. 2007. Microarray Analysis of Global Gene Expression of V. vinifera in Response to Xylella fastidiosa Infection. In: Proceedings of CDFA Pierce's Disease Control Program Research Symposium, December 12-14, 2007, San Diego, California. p. 254-257.

Interpretive Summary: In previous years we have dissected gene expression of Pierce’s disease (PD) resistant and susceptible genotypes of V. arizonica hybrids in response to infection by X. fastidiosa, the causal agent of PD. Here we report gene expression of the PD susceptible European grapevine, V. vinifera in response to X. fastidiosa infection. RNA extracted from healthy and infected leaf tissues was used for microarray based detection of differences in global gene expression. The results showed that there were a total of 2385 differentially expressed transcripts from early (4 week), middle (8 week) and late (10 week) stages of disease development. Of these 2385 transcripts, 1050 transcripts were up-regulated (2 to 100 fold) and 1335 transcripts were down-regulated (0.5 to 0.02 fold) across the three disease stages. In addition, comparative analysis of differentially expressed transcripts identified common and distinctive features of the host response from V. arizonica hybrids and V. vinifera to X. fastidiosa infection. An online relational database is now publicly available that has Vitis gene expression data along with other relevant information and bioinformatics tools. These results provide important knowledge for understanding the response of plant hosts to X. fastidiosa and the development of PD.

Technical Abstract: Previously, we analyzed gene expression profiles of Pierce’s Disease (PD) resistant and susceptible genotypes of V. arizonica hybrids in response to infection by X. fastidiosa (Xf). Here we report the gene expression profile of the PD susceptible European grapevine (V. vinifera) in response to Xf infection. RNA was extracted from healthy and infected leaf tissues at 4, 8, and 10 weeks post-inoculation. RNA isolated from plant samples was converted into cDNA and used for microarray-based global gene expression analyses. The data indicated that there were a total of 2385 differentially expressed transcripts from early (4 week), middle (8 week) and late (10 week) stages of disease development. Of these 2385 transcripts, 1050 transcripts were up-regulated (2 to 100 fold) and 1335 transcripts were down-regulated (0.5 to 0.02 fold) across the three disease stages compared to uninfected controls. In addition, comparative analysis of the differentially regulated transcripts identified common and distinctive features of the host response from V. arizonica hybrids and V. vinifera to X. fastidiosa infection that are important for understanding the PD resistant and susceptible mechanisms. An online relational database is now publicly available that has Vitis transcriptome data along with other relevant information and bioinformatics tools.

Last Modified: 10/20/2014
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