ARS | Publication request: Integrated and composite genome maps: the bovine example

Submitted to: Annual International Plant & Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: October 5, 2007
Publication Date: January 2, 2008
Citation: Snelling, W.M., Chiu, R., Schein, J.E., Hobbs, M., McEwan, J.C. 2008. Integrated and composite genome maps: the bovine example. Annual International Plant & Animal Genome Conference. Workshop Abstract W21, pg 9.

Technical Abstract: Combinations of genome maps representing different types of information are needed to link economically important phenotypic variation with underlying genomic variation in farmed animals. For the cow, data from two linkage populations and three radiation hybrid (RH) panels were combined to construct a composite marker map, exploiting the complimentary resolution of linkage and RH data. Fingerprints of clones from three bacterial artificial chromosome (BAC) libraries were analyzed to build a BAC physical map. BAC clone end sequences enabled integration of the composite and BAC maps with draft bovine and human sequence assemblies. Locations of published quantitative trait loci (QTL) were projected according to flanking marker positions on the composite map. The QTL indicate regions associated with specific traits, annotated human sequence provides information about genes which may influence each trait, and the bovine sequence provides a reference to known SNP as well as for detecting additional polymorphisms underlying the QTL. The combined resources may facilitate development of genomic tools to accelerate genetic improvement of cattle. The strategies used for the bovine maps may be useful to other livestock and aquatic species.