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United States Department of Agriculture

Agricultural Research Service

Research Project: NEW AND EXPANDED USES OF OILSEED PRODUCTS AND BY-PRODUCTS

Location: Commodity Utilization Research

Title: Modulation of multidrug resistance gene expression in human breast cancer cells by (-)-gossypol-enriched cottonseed oil.

Authors
item Ye, Weiping - COLLEGE OF VET. MEDICINE
item Chang, Hsiang-Lin - COLLEGE OF VET. MEDICINE
item Wang, Li-Shu - COLLEGE OF VET. MEDICINE
item Huang, Yi-Wen - COLLEGE OF VET. MEDICINE
item Shu, Sherry - COLLEGE OF VET. MEDICINE
item Dowd, Michael
item Wan, Peter
item Sugimoto, Yasuro - COL.VET.MED/OHIO STATE
item Lin, Young - COL.VET.MED/OHIO STATE

Submitted to: Anticancer Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: November 13, 2006
Publication Date: January 31, 2007
Citation: Ye, W., Chang, H-L., Wang, L-S. Huang, Y-W., Shu, S., Dowd, M.K., Wan, P.J., Sugimoto, Y., Lin, Y. 2007. Modulation of multidrug resistance gene expression in human breast cancer cells by (-)-gossypol-enriched cottonseed oil. Anticancer Research. 27:107-116.

Interpretive Summary: Experiments were conducted with (-)-gossypol-enriched cottonseed oil to demonstrate the potential of (-)-gossypol to inhibit drug resistant cancer cell lines. Addition of 0.05% of cottonseed oil to various therapeutic agents inhibited the growth of MCF-7/adr and PCHBCEC cells. The results will be of interest to researchers developing cancer therapies.

Technical Abstract: P-glycoprotein, the product of the multidrug resistance 1 gene, acts as an efflux pump and prevents sufficient intracellular accumulation of several anticancer agents. Thus, it plays a major role in multidrug cancer resistance. Using the non-radioactive cell proliferation MTS assay, none of three anticancer drug agents (Tamoxifen, ICI 182 780, and Adriamycin) inhibited MCF-7/adr (MDR human breast cancer) cells and only Adriamycin has a small inhibitory effect against PCHBCEC (primary cultured human breast cancer epithelial) cells. These same agents with 0.05% (-)-gossypol enriched cottonseed oil significantly inhibited MCF-7/adr cell growth by between 23 and 34%. For PCHBCE cells, the addition of (-)-gossypol enriched oil inhibited the cells by between 71 and 94%. The results demonstrate the multidrug-reversing potential of (-)-gossypol enriched cottonseed oil.

Last Modified: 9/21/2014
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