|Li, Ji - YALE UNIVERSITY SOM|
|Coven, David - YALE UNIVERSITY SOM|
|Miller, Edward - YALE UNIVERSITY SOM|
|Hu, Xiaoyue - YALE UNIVERSITY SOM|
|Carling, David - IMPERIAL COLLEGE SOM UK|
|Sinusas, Albert - YALE UNIVERSITY SOM|
|Young, Lawrence - YALE UNIVERSITY SOM|
Submitted to: American Journal of Physiology - Heart and Circulatory Physiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: April 26, 2006
Publication Date: April 28, 2006
Citation: Li, J., Coven, D.L., Miller, E.J., Hu, X., Young, M.E., Carling, D., Sinusas, A.J., Young, L.H. 2006. Activation of AMPK alpha and gamma-isoform complexes in the intact ischemic rat heart. American Journal of Physiology - Heart and Circulatory Physiology. 291:H1927-H1934. Interpretive Summary: During a myocardial infarction (heart attack) the heart muscle becomes deprived of oxygen and nutrients. As such, large changes in metabolism occur, allowing the heart to produce adequate amounts of usable energy for continued heart function (and therefore sustained life of the organism). A family of proteins that likely play a central role in metabolic changes observed during and following myocardial infarction are AMP-activated protein kinases (AMPK). This study therefore investigated the effects of simulated myocardial infarction conditions on different AMPK family members. The results suggest that different members of AMPK complexes are distinctly regulated in the heart during simulated myocardial infarction conditions.
Technical Abstract: AMP-activated protein kinase (AMPK) plays a key role in modulating cellular metabolic processes. AMPK, a serine-threonine kinase, is a heterotrimeric complex of catalytic alpha-subunits and regulatory beta- and gamma-subunits with multiple isoforms. Mutations in the cardiac gamma(2)-isoform have been associated with hypertrophic cardiomyopathy and pre-excitation syndromes. However, physiological regulation of AMPK complexes containing different subunit isoforms is not well defined and is important for an understanding of the function of this signaling pathway in the intact heart. We evaluated the kinase activity associated with heart AMPK complexes containing specific alpha- and gamma-subunit isoforms of AMPK in an in vivo rat model of regional ischemia. Left coronary artery occlusion activated the immunoprecipitated alpha(1)-isoform (6-fold, P < 0.01) and alpha(2)-isoform (9-fold, P < 0.01) in the ischemic left ventricle compared with sham controls. The degree of alpha-subunit activation depended on the extent of ischemia and paralleled echocardiographic contractile dysfunction. The regulatory gamma(1)- and gamma(2)-isoforms were expressed in the heart. The gamma(1)- and gamma(2)-isoforms coimmunoprecipitated with alpha(1)- and alpha(2)-isoforms in proportion to alpha-subunit content. gamma(1)-Isoform immunocomplexes accounted for 70% of AMPK activity and AMPK phosphorylation (Thr(172)) in hearts. Ischemia similarly increased AMPK activity associated with the gamma(1)- and gamma(2)-isoform complexes threefold (P < 0.01 for each). Thus AMPK catalytic alpha(1)- and alpha(2)-isoforms are activated by regional ischemia in vivo in the heart, irrespective of the regulatory gamma(1)- or gamma(2)-isoforms to which they are complexed. Despite the pathophysiological importance of gamma(2)-isoform mutations, gamma(1)-isoform complexes account for most of the AMPK activity in the ischemic heart.