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Title: DETECTION OF PATHOGENS BY FLOURESCENCE SPECTROSCOPY

Author
item Sohn, Mi Ryeong
item Himmelsbach, David
item Barton Ii, Franklin

Submitted to: Proceedings of Pittcon Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 7/27/2007
Publication Date: 3/1/2008
Citation: Sohn, M., Himmelsbach, D.S., Barton Ii, F.E. 2008. Detection of pathogens by flourescence spectroscopy. Proceedings of Pittcon Meeting. New Orleans, March 2-7, 2008. Abstract (#870-26p).

Interpretive Summary:

Technical Abstract: Abstract This study was performed as a preliminary study for rapid detection of food bone pathogens by fluorescence spectroscopy. E coli, Salmonella and Campylobactor which are the most commonly found in food were used in this study. Bacteria were grown in agar plate and diluted in saline and prepared in different concentrations. The bacteria samples were loaded in quartz cell and scanned in the range of 200-700 nm using a fluorescence spectrometer. The excitation and emission slits were 5 nm and scan speed used was 240 nm/min. Synchronous scanning was employed to find the maximum excitation and emission wavelengths for each bacterium. The maximum emission was observed in 340-350 nm with excitation at 220 and 280 nm. The fluorescence spectra for three bacteria were very similar, indicating difficulty of classification of three pathogens. Multivariate data treatment using principal component analysis and preprocessing of the spectral data allowed them to separate clearly according to their types and concentrations.