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United States Department of Agriculture

Agricultural Research Service

Title: Secreted maltase-glucoamylase is upregulated in membrane maltase-glucoamylase null mice

Authors
item Nichols, B - BAYLOR COLLEGE MED
item Avery, S - BAYLOR COLLEGE MED
item Sen, P - BAYLOR COLLEGE MED
item Robayo, C - BAYLOR COLLEGE MED
item Quezada-Calvillo, R - UNIV. SAN LUIS POTOSI
item Wattler, S - INGENIUM PHARM. GERMANY
item Nehls, M - INGENIUM PHARM. GERMANY
item Lugenbuehl, U - UNIV. BERN
item Sterchi, Erwin - UNIV. BERN

Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: June 1, 2005
Publication Date: October 1, 2005
Citation: Nichols, B.L., Avery, S., Sen, P., Robayo, C., Quezada-Calvillo, R., Wattler, S., Nehls, M.C., Luginbuehl, U., Sterchi, E. 2006. Secreted maltase-glucoamylase is upregulated in membrane maltase-glucoamylase null mice. In: Naim HY, Zimmer KP, editors. The Brush Border Membrane: From Molecular Cell Biology to Clinical Pathology. Heilbronn, Germany: SPS Verlagsgesellschaft. p. 214-224.

Technical Abstract: The known mammalian brush-border glucohydrolases are membrane-bound, small intestine lumenal enzymes that digest dietary starches and sugars into monosaccharides for absorption. The phenotype of maltase-glucoamylase deficiency is not understood. To address this, we ablated membrane-bound maltase-glucoamylase (Mgamme) in the mouse. This was accomplished with a cassette that inserted a stop codon within exon 2, the membrane-binding domain. Here we report the discovery of a novel soluble maltase-glucoamylase peptide (Mgamso). Membrane bound maltase-glucoamylase was absent by Western blot in Mgamme null mice, but soluble maltase-glucoamylase activity was present in both small and large bowel of membrane maltase-glucoamylase null mice. Sequencing of small intestinal cDNA from Mgamme null mice confirmed absence of maltase-glucoamylase exon 2. Sequencing of cDNA from Mgamme wild type mice revealed a low level of the 1,3 spliceoform message. The copy number of the 1,3 spliceoform was increased by 8 fold in the Mgamme null mouse.

Last Modified: 12/20/2014
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