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United States Department of Agriculture

Agricultural Research Service

Research Project: NOVEL TECHNOLOGIES AND TECHNIQUES FOR THE DETECTION OF RESIDUES, TOXINS, AND OTHER CHEMICALS IN FOODS

Location: Residue Chemistry and Predictive Microbiology

Title: Large Volume Injection Techniques in Capillary Gas Chromatography

Authors
item Hoh, Eunha
item Mastovska, Katerina

Submitted to: Journal of Chromatography A
Publication Type: Review Article
Publication Acceptance Date: December 3, 2007
Publication Date: December 8, 2007
Citation: Hoh, E., Mastovska, K. 2007. Large Volume Injection Techniques in Capillary Gas Chromatography. Journal of Chromatography A [serial online]. 10.1016. Available: http://dx.doi.org/10.1016/j.chroma.2007.12.001.

Technical Abstract: Large volume injection (LVI) is a prerequisite of modern gas chromatographic (GC) analysis, especially when trace sample components have to be determined at very low concentration levels. Injection of larger than usual sample volumes increases sensitivity and/or reduces (or even eliminates) the need for extract concentration steps. Also, an LVI technique can serve as an interface for on-line connection of GC with a sample preparation step or with liquid chromatography. This article reviews the currently available LVI techniques, including basic approaches to their optimization and important real-world applications. The most common LVI methods are on-column and programmed temperature vaporization (PTV) in solvent split mode. Newer techniques discussed in this article include direct sample introduction (DSI), splitless overflow, AT-column, and “through oven transfer adsorption desorption” (TOTAD).

Last Modified: 8/27/2014
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