Skip to main content
ARS Home » Midwest Area » Urbana, Illinois » Soybean/maize Germplasm, Pathology, and Genetics Research » Research » Publications at this Location » Publication #213879

Title: Polymorphism of the Hsp90 Gene among Populations of Heterodera glycines from China, Japan, and the United States

Author
item ATIBALENTJA, N - UNIV OF ILLINOIS
item Noel, Gregory

Submitted to: Journal of Nematology
Publication Type: Abstract Only
Publication Acceptance Date: 7/29/2007
Publication Date: 8/1/2007
Citation: Atibalentja, N., Noel, G.R. 2007. Polymorphism of the Hsp90 Gene among Populations of Heterodera glycines from China, Japan, and the United States. Journal of Nematology.39(1):94-95 Mar2007

Interpretive Summary:

Technical Abstract: Hsp90 is a member of a gene family that codes for proteins whose synthesis is enhanced by a variety of environmental stressors, including a sudden increase in temperature. These proteins function as molecular chaperones in cells where they interact with a range of regulatory proteins involved in critical physiological processes such as cell cycle control and hormone signaling. Recently, the complete protein sequence and a partial genomic sequence of the Heterodera glycines homolog of Hsp90 (Hg-hsp90) were reported. In this study, we cloned the complete genomic sequence of Hg-hsp90 and investigated the extent of polymorphism of this gene among 16 populations of H. glycines from China (5), Japan (5), and the U.S. (6). The genomic clone of Hg-hsp90 was 3,052-nt long and, like that of the filarial nematode Brugia pahangi, contained 11 introns, compared with only 3 in Caenorhabditis elegans. The estimates of gene diversity among populations from China (0.64%), Japan (0.65%), and the U.S. (0.61%) were not significantly different. However, the extent of variation observed in this study is similar to that obtained with the ITS-rRNA gene, which shows that Hg-hsp90, a single or low copy number gene, unlike the multi-copy ITS-rRNA gene, is a better candidate for nematode phylogenetics.