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United States Department of Agriculture

Agricultural Research Service

Research Project: BIOLOGICAL CONTROL OF INSECTS AND MICROORGANISMS TO PREVENT MYCOTOXIN CONTAMINATION

Location: Foodborne Toxin Detection and Prevention

Title: On-Plant Volatile Analysis Utilizing Solid-Phase Microextraction and a New Volatile Collection Technique

Authors
item BECK, JOHN
item Merrill, Glory
item Higbee, Bradley - PARAMOUNT FARMING CO.

Research conducted cooperatively with:
item Paramount Farming Company, Llc

Submitted to: American Society of Pharmacognosy
Publication Type: Abstract Only
Publication Acceptance Date: May 16, 2007
Publication Date: July 14, 2007
Citation: Beck, J.J., Merrill, G.B., Higbee, B.S. 2007. On-Plant Volatile Analysis Utilizing Solid-Phase Microextraction and a New Volatile Collection Technique. American Society of Pharmacognosy. Meeting Abstract.

Interpretive Summary: Volatile emission of plants is typically measured by removal of the plant-part, transportation to a laboratory, and subsequent volatile analyses via a number of accepted methodologies. Studies performed by our laboratory have shown the volatile emission of removed plant parts are essentially identical to the volatiles produced if the plants parts were injured. Using a new method of on-plant volatile collection the volatile output of the readily available Pittosporum undulatum fruit was measured before and after injury while the fruit was still intact on the tree. In a corresponding experiment, the volatile emission of an intact fruit was evaluated then the fruit removed from its host and the volatile output of the fruit reevaluated. The results demonstrated the volatiles produced by the damaged fruit were essentially identical to the volatile emission of the removed fruit. In addition, results from two other studies utilizing this new technique will be presented.

Technical Abstract: Volatile emission of plants is typically measured by removal of the plant-part, transportation to a laboratory, and subsequent volatile analyses via a number of accepted methodologies. Studies performed by our laboratory have shown the volatile emission of removed plant parts are essentially identical to the volatiles produced if the plants parts were injured. Using a new method of on-plant volatile collection the volatile output of the readily available Pittosporum undulatum fruit was measured before and after injury while the fruit was still intact on the tree. In a corresponding experiment, the volatile emission of an intact fruit was evaluated then the fruit removed from its host and the volatile output of the fruit reevaluated. The results demonstrated the volatiles produced by the damaged fruit were essentially identical to the volatile emission of the removed fruit. In addition, results from two other studies utilizing this new technique will be presented.

Last Modified: 9/10/2014
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