Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: September 26, 2007
Publication Date: December 13, 2007
Citation: Li, W., Adkins, S.T., Hilf, M.E. 2007. Characterization of complete sequences of RNA 1 and RNA 2 of citrus variegation virus. Archives of Virology. 153:385-388. Interpretive Summary: Citrus variegation virus (CVV) is a graft and mechanically tranmissible viral pathogen of citrus. Until the work described in this paper was completed, the genetic sequence of CVV was only partially known. This manuscript describes the completion of the genetic sequence of CVV. The completed sequence provides information which can be used to synthesize effective reagents for CVV detection and control.
Technical Abstract: Citrus variegation virus (CVV), a member of the genus Ilarvirus, was originally isolated from citrus but was reported to infect many herbaceous plants under greenhouse conditions. The virus particles are approximately 30 nm in diameter, and are composed of coat proteins (CP) with a Mr of 26 kDa, and five RNA species designated as RNAs 1, 2, 3, 4 and 4a, respectively. A very close serological relationship was found between CVV and Citrus leaf rugose virus (CLRV), a member of Ilarvirus subgroup 2. Both putative translation products of the complete sequence of CVV RNA 3 of (GenBank accession U17389) and of the partial ORF from the partial sequence of RNA 2 (GenBank accession U93605) were closely related to Elm mottle virus (EMoV), another member of subgroup 2. These two pieces of evidence suggested CVV be placed into subgroup 2 of the genus Ilarvirus. To date, the sequence for CVV RNA 3 (2309 nt) has been completed, but only partial sequences for CVV RNAs 1 (GenBank accession U93604) and 2 are available. This report presents the complete sequences of CVV RNA 1 and RNA 2. Phylogenetic analysis of the putative replicase and polymerase proteins of CVV and the corresponding proteins of other ilarviruses supports placement of CVV as a member of Ilarvirus subgroup 2.