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ARS Home » Midwest Area » Ames, Iowa » Corn Insects and Crop Genetics Research » Research » Publications at this Location » Publication #211345
Title: POSITIONING THREE QUALITATIVE TRAIT LOCI ON SOYBEAN MOLECULAR LINKAGE GROUP E

Author
item Palmer, Reid
item MIN, X - ISU

Submitted to: Journal of Heredity
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/24/2008
Publication Date: 9/8/2008
Citation: Palmer, R.G., Min, X. 2008. Positioning Three Qualitative Trait Loci on Soybean Molecular Linkage Group E. Journal of Heredity. 99:674-678.

Interpretive Summary: Genetic traits can serve as landmarks on a roadmap for soybean. Some traits have agronomic importance, other traits such as chlorophyll-deficiency or yellow leaves may only be of academic interest. Two yellow-leaf mutants showed association (linkage) to a pubescence (‘hair’ or trichome) trait on soybean leaves, stems, and pods. Also a male-sterile, female-fertile mutant was reported to be loosely associated with the two yellow mutants. Our objective was to determine, 1) the location of each yellow mutant in relation to the pubescence trait, and 2) the location of the two yellow mutants to each other. Both yellow mutants were loosely associated with the pubescence trait and the distance from this trait was approximately the same for both yellow mutants. There was very close association of the two yellow mutants with each other. The significance of this research is that our data suggests that both yellow mutants were derived from one ancestral yellow mutant. That is the two derived yellow mutants initially were identical, but through evolution have changed to different chlorophyll-deficiency features. Our results should help molecular biologists in their studies of trait duplication in soybean.

Technical Abstract: In soybean [Glycine max (L.) Merr.] three qualitative trait loci (Pb, Y9, and Y17) are located on classical linkage group 14, which corresponds to molecular linkage group E. The Pb locus conditions sharp/blunt pubescence tip; the y9 and y17 loci condition green/chlorotic foliage. The gene order is not known. The objective was to determine the gene order on soybean molecular linkage group E of the Pb, Y9, and Y17 loci using previously mapped simple sequence repeat markers. Allelism tests between y9 and y17 gave normal green foliage F1 plants, indicating non-allelism. Our F2 data from the allelism test could not distinguish between a 1:1 or a 9:7 ratio. The F2:3 family segregation indicated a very close genetic linkage between the y9 and y17 loci. Two molecular mapping populations were developed. Population-1 segregated for Pb and y9 and population-2 segregated for Pb and y17. The gene order on soybean molecular linkage group E, using simple sequence repeat markers was Pb, Y9, Y17.