ARS | Publication request: Application of Fluorescence Spectroscopy for Rapid Detection of Pathogens in Food

Submitted to: Eastern Analytical Symposium
Publication Type: Abstract Only
Publication Acceptance Date: April 9, 2007
Publication Date: November 1, 2007
Citation: Sohn, M., Himmelsbach, D.S., Barton Ii, F.E. 2007. Application of Fluorescence Spectroscopy for Rapid Detection of Pathogens in Food. Eastern Analytical Symposium. Abstract #441. p. 62. 2007.

Technical Abstract: The potential of fluorescence spectroscopy was investigated for the detection food bone pathogens. E coli, Salmonella and Campylobactor, the most commonly present in food, were selectively identified. Each pathogen, grown in agar plate, was diluted in saline and prepared in different concentrations. Samples were loaded in quartz cell and scanned in the range of 200-700 nm using a fluorescence spectrometer. The excitation and emission slits were 5 nm and scan speed used was 240 nm/min. Synchronous spectra showed that all bacteria samples have an emission maximum at 340-350 nm with excitation at 220 or 280 nm. The fluorescence spectra were very similar in three bacteria. However, multivariate data treatment using principal component analysis (PCA) resulted in clear classification of bacteria according to their types as well as their concentrations.