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ARS Home » Midwest Area » Ames, Iowa » Corn Insects and Crop Genetics Research » Research » Publications at this Location » Publication #210757
Title: The Male Sterility Locus ms3 is Present in a Fertility Controlling Gene Cluster in Soybean

Author
item CERVANTES-MARTINEZ, INNAN - ISU
item XU, M - ISU
item ORTIZ-PEREZ, E - ISU
item KATO, K - OBIHIRO UNIV JAPAN
item HORNER, H - ISU
item Palmer, Reid

Submitted to: Journal of Heredity
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/23/2009
Publication Date: 7/4/2009
Citation: Cervantes-Martinez, I., Xu, M., Ortiz-Perez, E., Kato, K.K., Horner, H.T., Palmer, R.G. 2009. The Male Sterility Locus ms3 is Present in a Fertility Controlling Gene Cluster in Soybean. Journal of Heredity. 10:565-570.

Interpretive Summary: Many field crops, horticultural crops, and forest tree crops are hybrids. In addition to hybrid vigor of the plants, there is a 'built-in' intellectual property advantage with the hybrid annual crops. Sterility systems, e.g., male-sterile, female-fertile plants, can be used as the mother parent to produce hybrids. For some plant breeding methods, it is important to know the genetic location of the sterility trait. Our objective was to locate the male-sterile, female-fertile ms3 trait. This trait is used in soybean to produce experimental quantities of hybrid seed. The location is like a roadmap. The ms3 trait was located in a region with two other fertility/sterility traits. The significance of this observation, if any, is not known. With the location of ms3 known, it can be genetically 'moved' by breeders more efficiently in soybean cultivar improvement programs. Improved cultivars benefit the seed producer (company), the farmer, and ultimately the consumer, through lower costs because of greater efficiency by the plant breeder.

Technical Abstract: Soybean [Glycine max (L.) Merr.] is a self-pollinated plant. Manual cross-pollination is used to produce limited quantities of hybrid seed. To produce large quantities of hybrid seed, insect-mediated cross-pollination is necessary. An efficient nuclear male-sterile system for hybrid seed production would benefit from molecular and/or phenotypic markers linked to the male fertility/sterility locus to facilitate early identification of the phenotypes. Nuclear male-sterile, female-fertile ms3 mutant is inherited as a single recessive gene, and displays high out-crossed seed-set in the presence of insect pollinators. The objective of this study was to map the ms3 locus to a chromosome location and to define the genetic distances between markers. A segregating F2 population was developed from the cross of cultivar Minsoy (PI 27890) x T284H, Ms3ms3 (A00-68). The mapping population consisted of 150 F2 plants that were screened using a total of 231 simple sequence repeat (SSR) markers, with a criterion for selection of 25 cM between each marker. The ms3 locus was mapped to molecular linkage group (MLG) D1b between markers Satt157 and Satt542, with a distance of 3.7 and 12.3 cM, respectively. Female-partial sterile 1 (Fsp1 from Clark-k2) mutant and the Midwest Oilseed male-sterile (msMOS) mutant previously were located on MLG D1b. A cross was made between msMOS and Clark-k2 and the recombination rate between the msMOS and Fsp1 loci was 9.04%. An integrated map showed a distance between the ms3 and Fsp1 loci of 15.0 cM, and the Join Map of the ms3, Fsp1, and msMOS loci demonstrated that all three loci were positioned in the same chromosomal region on MLG D1b.