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United States Department of Agriculture

Agricultural Research Service

Title: Molecular cloning of an SSR marker associated with resistance to Sclerotinia blight in peanut and sequence variation among resistant and susceptible plant lines

Author
item Chamberlin, Kelly

Submitted to: American Peanut Research and Education Society Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: May 1, 2007
Publication Date: July 12, 2007
Citation: Chenault, K.D. 2007. Molecular cloning of an SSR marker associated with resistance to Sclerotinia blight in peanut and sequence variation among resistant and susceptible plant lines [abstract]. In: Proceedings of the American Peanut Research and Education Society Proceedings, July 10-13, 2007, Birmingham, Alabama. 39:59. Available: http://www.apresinc.com/pdf/Proceedings/Volume%2039,%20Proceedings.pdf.

Technical Abstract: The production of cultivated peanut, an important agronomic crop throughout the United States and the world, is consistently threatened by various diseases and pests. Specifically, peanut production in Oklahoma, Texas, North Carolina, and Virginia is challenged by fungal disease such as Sclerotinia blight. The identification of a molecular marker associated with fungal resistance in peanut would greatly assist peanut geneticists in selecting genotypes to be used in breeding programs. Using simple sequence repeat (SSR) primers reported for peanut, molecular markers were identified which are associated with reaction to the fungus Sclerotina minor, the causal agent of Sclerotinia blight. Two markers, one consistent with resistance and the other with susceptibility, have been cloned and sequenced from several different peanut genotypes. Sequence analysis revealed that both markers are from the same region on the peanut genome that is well conserved except for differences surrounding the tandem repeat sequence, which varies in length depending on genotype. Studies are underway to understand the nature and significance of these differences which will further illustrate the utility of these markers. Future use of these markers to screen segregating populations and/or germplasm collections will greatly enhance the efficiency of breeding peanut with resistance to Sclerotinia blight.

Last Modified: 10/1/2014
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