MOLECULAR CHARACTERIZATION AND DIVERSITY ASSESSMENT OF COCOA GERMPLASM IN THE AMERICAS
Title: Differential gene expression between the biotrophic-like and saprotrophic mycelia of the Witches’ broom pathogen Moniliophthora perniciosa.
| Rincones, Johana - UNIV OF CAMPINAS, BRAZIL |
| Scarpari, Leandra - UNIV OF CAMPINAS, BRAZIL |
| Mondego, Jorge - UNIV OF CAMPINAS, BRAZIL |
| Formighieri, Eduardo - UNIV OF CAMPINAS, BRAZIL |
| Sabha, Maricene - UNIV OF CAMPINAS, BRAZIL |
| Carazzolle, Marcelo - UNIV OF CAMPINAS, BRAZIL |
| Barau, Joan - UNIV OF CAMPINAS, BRAZIL |
| Pereira, Goncalo - UNIV OF CAMPINAS, BRAZIL |
Submitted to: Fungal Genetics Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: December 1, 2007
Publication Date: March 20, 2008
Citation: Rincones, J., Scarpari, L.M., Mondego, J.M., Formighieri, E.F., Sabha, M., Carazzolle, M.F., Barau, J.G., Meinhardt, L.W., Pereira, G.A. Differential gene expression between the biotrophic-like and saprotrophic mycelia of the Witches’ broom pathogen Moniliophthora perniciosa. Fungal Genetics Conference Proceedings. www.fgsc.net/asil2007/xxivfgcposterabs.htm
In order to improve our understanding of the molecular mechanisms involved in the biotrophic development of M. perniciosa, we performed DNA microarrays and EST analyses of the biotrophic and saprotrophic stages of this fungus, grown in vitro. Reads from 3 cDNAs libraries (glucose- and cacao-induced saprotrophic mycelia and cacao-induced biotrophic mycelium) were clusterized into 1596 unigenes. DNA microarray analysis was performed for 2304 genomic DNA fragments, with probes prepared from mRNA of the biotrophic and saprotrophic mycelia. Our results suggest that both types of mycelia show different carbon metabolisms, with glycolysis and the glyoxalate shunt being repressed in the biotrophic phase. Moreover, the biotrophic stage showed induced transcripts indicative of amino acid starvation, as well as several characterized as pathogenicity factors, such as Glyoxal oxidase, ceratoplatanin, proteases and peroxidases. Differential expression of selected genes was confirmed by Real Time-PCR. This work is the first to analyze global gene expression between the infective and saprotrophic stages of a hemibiotrophic fungus.