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Title: Mechanisms of Virus Adsorption Following Land Application of Anaerobically Treated Flushed Dairy Manure Wastewater

Author
item Davis, Johnnie
item FARRAH, SAMUEL - UNIVERSITY OF FLORIDA
item WILKIE, ANN - UNIVERSITY OF FLORIDA

Submitted to: American Society for Microbiology Conference
Publication Type: Abstract Only
Publication Acceptance Date: 2/16/2007
Publication Date: 5/24/2007
Citation: Davis, J.A., Farrah, S.R., Wilkie, A.C. 2007. Mechanisms of Virus Adsorption Following Land Application of Anaerobically Treated Flushed Dairy Manure Wastewater. American Society for Microbiology Conference. 266/Q(Q-327):603.

Interpretive Summary:

Technical Abstract: The soil adsorption mechanisms of viruses in untreated and anaerobically treated animal manure wastewater and groundwater was investigated. Batch adsorption studies were performed using cationic (hexadecyltrimethylammonium bromide, HTAB), anionic (sodium dodecyl sulfate, SDS), and nonionic (polyoxyethylene sorbitan monooleate, Tween 80) detergents. In groundwater, there was a small (< 10%) increase in adsorption of MS2 to soil in the presence of Tween 80 and SDS. However, PRD1 adsorption to soil increased > 50% in the presence of Tween 80 and SDS. PhiX174 adsorption to soil decreased by 60% in the presence of Tween 80, but remained unchanged in the presence of SDS. Adsorption of MS2 in anaerobically treated wastewater with Tween 80 and SDS was similar to groundwater results. However, in untreated wastewater, adsorption of MS2 increased by 41% in the presence of Tween 80. Adsorption of PRD1 increased > 75% in the presence of Tween 80 and SDS in untreated wastewater. Adsorption of PhiX174 remained unchanged in the presence of Tween 80 and SDS in untreated wastewater. HTAB increased adsorption of all viruses in groundwater and wastewaters to soil by > 99%. Soil pretreated with Tween 80 in groundwater decreased adsorption of MS2 and PRD1 by 36% and 26%, respectively. However, SDS in groundwater caused a 23% reduction in adsorption of MS2 while PRD1 remained unchanged. Adsorption of PhiX174 decreased slightly (< 10%) in soil pretreated with Tween 80 and SDS. Adsorption increased to > 99% for all viruses in soil pretreated with HTAB, suggesting strong adsorption of viruses to soil in the presence of cations. Furthermore, elution of MS2 and PhiX174 from soil using Tween 80 and SDS was < 20% (no significant difference, P < 0.05). However, elution of PRD1 from soil using Tween 80 was > 30%. These results demonstrate that adsorption of MS2 and PhiX174 in untreated and anaerobically digested wastewater and groundwater to soil is complex. Also, adsorption of PRD1 to soil appears to occur via hydrophobic interactions.