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ARS Home » Northeast Area » Kearneysville, West Virginia » Appalachian Fruit Research Laboratory » Innovative Fruit Production, Improvement, and Protection » Research » Publications at this Location » Publication #209460

Title: Transcript initiation in three peach (Prunus persica L., Batsch.) dehydrins: tissue specificity differences in two promoters

Author
item Bassett, Carole
item Artlip, Timothy - Tim
item RICHART, GREGORY - PENNSYLVANIA STATE UNIV
item Wisniewski, Michael
item FARRELL, ROBERT - PENNSYLVANIA STATE UNIV

Submitted to: HortScience
Publication Type: Abstract Only
Publication Acceptance Date: 11/2/2006
Publication Date: 7/16/2007
Citation: Bassett, C.L., Artlip, T.S., Richart, G.A., Wisniewski, M.E., Farrell, R.E. 2007. Transcript initiation in three peach (Prunus persica L., Batsch.) dehydrins: tissue specificity differences in two promoters. HortScience.

Interpretive Summary:

Technical Abstract: Plants must adapt to environmental stresses to survive. As a result, they have evolved numerous strategies to reduce stress-induced damage, ranging from adaptive morphological features to changes in gene expression which protect cellular components. A family of genes known as dehydrins has a role in stress protection specifically related to cold, dehydration and salt exposure. We have been analyzing the expression of three dehydrin family members in peach, PpDhn1-3. All three genes are expressed in various tissues in the order bark > leaf > xylem > root > fruit. In addition, we have previously demonstrated that PpDhn1 and 3 are primarily responsive to cold, whereas, PpDhn2 is primarily responsive to water deficit. Here, we present the results of RNA Ligase-Mediated Rapid Amplification of cDNA Ends (RLM-RACE) to identify the transcription start sites for these three genes in bark and fruit tissues. The results indicate that PpDhn1 and 2 utilize different transcription start sites in developing fruit compared to bark sampled in July or December, whereas, PpDhn3 initiates at the same site in bark and developing fruit.