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ARS Home » Southeast Area » Stoneville, Mississippi » Crop Genetics Research » Research » Publications at this Location » Publication #209126
Title: Protein SDS-PAGE Characterization and Comparison of Soybean Cyst Nematode

Author
item Donald, Patricia
item PATANANAN, ALEXANDER - BATTELLE NORTHWEST
item SIMMONS, TERE - BATTELLE NORTHWEST
item CASTRO, NATHAN - BATTELLE NORTHWEST
item WILGAR, JACULINE - BATTELLE NORTHWEST
item GOHEEN, STEVEN - BATTELLE NORTHWEST

Submitted to: Nematology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/20/2008
Publication Date: 12/1/2008
Citation: Donald, P.A., Patananan, A.N., Simmons, T., Castro, N., Wilgar, J.L., Goheen, S.C. 2008. Protein SDS-PAGE Characterization and Comparison of Soybean Cyst Nematode. Nematology. 38(2):137-144.

Interpretive Summary: Soybean cyst nematode (SCN, Heterodera glycines Ichinohe) represents one of the most serious threats to the stability of soybean production in the United States. Soybean producers reduce their risk of yield loss by planting soybean varieties which are resistant to certain populations of the nematode. Use of resistant varieties has selected SCN populations which are able to reproduce on different sources of plant resistance making those sources of resistance no longer effective in preventing yield loss. Differences in the nematode proteins were examined as a correlation with virulence. Examination of 25 major proteins revealed no association of these proteins with nematode virulence. Thus scientists need to find other methods for distinguishing variability among SCN populations to aid producers in selection of varieties to plant.

Technical Abstract: Soybean cyst nematode (SCN, Heterodera glycines Ichinohe) represents one of the most serious threats to the stability of soybean production in the United States. Use of plant resistance has selected SCN populations which are able to reproduce on different sources of plant resistance. Producers must identify variability in these populations in fields when selecting varieties for planting, but soil sampling and population identification is expensive and extrapolation from the sample to the entire field is problematic. Variability in populations is not associated with any morphological differences among populations, but knowledge of biochemical differences among populations is lacking. Therefore, the protein profiles of five different SCN populations were characterized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in order to identify similarities and differences in their most prominent proteins. No major biochemical differences in 25 proteins ranging in molecular weight from 40 to 200 kDa were observed among these populations. These results suggest that SCN populations cannot be differentiated by these proteins and that other methods for distinguishing variability among populations are needed.