ARS | Publication request: Molecular Characterization of Globodera pallida associated with potato in Idaho

Submitted to: Society of Nematologists Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: March 13, 2007
Publication Date: September 28, 2007
Citation: Skantar, A.M., Carta, L.K., Handoo, Z.A., Chitwood, D.J. 2007. Molecular Characterization of Globodera pallida associated with potato in Idaho [abstract]. Joint Meeting of The American Phytopathological Society and Society of Nematologists. p. 30.

Technical Abstract: Molecular diagnostic methods were used to positively identify a new population of pale potato cyst nematode Globodera pallida (Stone) Behrens. PCR-RFLP of the rDNA ITS region, sequence-specific multiplex PCR, and DNA sequence comparisons all confirmed the identity of the Idaho population as G. pallida. The ITS rDNA of the Idaho isolate was identical to sequences obtained for populations from York, England and the Netherlands. Because the tobacco cyst nematode Globodera tabacum and the similar golden potato cyst nematode G. rostochiensis are also present in the U.S., the ability to distinguish G. pallida from these species will be particularly important as more states perform surveys for these cyst nematodes. To prepare for the possibility of mixed field populations, we have designed species-specific primers that can discriminate G. tabacum from G. pallida and G. rostochiensis. Future prospects for additional molecular diagnostics will also be discussed.