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United States Department of Agriculture

Agricultural Research Service

Research Project: IDENTIFICATION AND CHARACTERIZATION OF PEST INSECT IMMUNE RESPONSES TO BIOLOGICAL CONTROL AGENTS

Location: Biological Control of Insects Research

Title: Genomic sequence analysis of a nucleopolyhedrovirus isolated from the fall armyworm, Spodoptera frugiperda

Authors
item Popham, Holly
item Harrison, Robert

Submitted to: American Society for Virology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: April 4, 2007
Publication Date: June 19, 2007
Citation: Popham, H.J., Harrison, R.L. 2007. Genomic sequence analysis of a nucleopolyhedrovirus isolated from the fall armyworm, Spodoptera frugiperda. American Society for Virology. p. 259.

Technical Abstract: The complete genomic sequence of this Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) clone was determined and analyzed from a field isolate collected in Missouri, USA. The SfMNPV genome was 131,330 bp with a 40.2% G+C content. Eight homologous repeat regions (hrs) were identified that consisted of 43-bp imperfect palindromes with significant sequence similarity to the hr repeats of Spodoptera exigua MNPV and Agrotis segetum NPV. One hundred and forty-four ORFs were identified, including the set of 62 genes found among all baculovirus genomes. Comparison of ORF content, order, and predicted amino acid sequences with other nucleopolyhedoviruses indicated that SfMNPV is part of a cluster of viruses within NPV group II that includes NPVs from Spodoptera, Agrotis, and Mamestra host species. SfMNPV-Missouri shared a high degree of nucleotide sequence similarity with other SfMNPV isolates that have been partially sequenced.

Last Modified: 7/22/2014
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