CONTROL OF GAMMAHERPESVIRUS-ASSOCIATED MALIGNANT CATARRHAL FEVER IN RUMINANTS
Location: Animal Diseases Research
Title: Characterization of Bison bison major histocompatibitity complex class I haplotypes
Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: November 15, 2006
Publication Date: December 3, 2006
Citation: Davies, C.J., Traul, D., New, D.D., Taus, N.S., O'Toole, D., Li, H. 2006. Characterization of Bison bison major histocompatibitity complex class I haplotypes. Research Workers in Animal Diseases Conference Proceedings, Chicago, Illinois. Pg. 156.
American bison (Bison bison) are highly susceptible to malignant catarrhal fever (MCF). Nevertheless, 20% of clinically normal bison have antibodies to ovine berpesvirus-2 (OvHV-2), the etiologic agent of MCF in North America. The bison MHC class II (MHC-II) genes were characterized in a previous study (Traul et al., Immunogenetics 57:845-854, 2005). We also found that bison DRB3 alleles were associated with susceptibility to MCF. However, the linked MHC-I genes could be responsible for the DRB3 disease association. The objective of this study was to sequence the expressed classical MHC-I genes from as many bison MHC-I haplotypes as possible. Twenty-seven bison carrying 15 DRB3 alleles were selected on the basis of microarray DRB typing (Park et al., J Vet Sci 5:29-39, 2004). For each bison, cDNA was amplified using primers for conserved sites in exon I and the 3' UTR, cloned into the pCR4-TOPO vector and 48 to 96 clones sequenced by cycle sequencing (Davies et al. Am J Reprod Immunol 55:188-200, 2006). Thirty-six expressed classical MHC-I alleles were identified in the 27 bison. The alleles were assigned to five loci based on phylogenetic and haplotype analyses. One cattle and 23 bison MHC-I haplotypes were identified. The cattle haplotype carried alleles BoLA-N8*00201 and BoLA-N*01201 and was associated with a cattle MHC-II haplotype (BoLA.DH22F). None of tile MHC-I loci were expressed in all haplotypes. The loci were expressed in different combinations: four haplotypcs had one, 14 had two, four had three and one had four expressed classical MHC-I genes.