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Title: Application of bacteriocin produced by Streptococcus cricetus S760 for treating Campylobacter jejuni and Salmonella-associated infections in broilers

Author
item SVETOCH, E - ST RES CTR RUSSIA
item ERUSLANOV, B - ST RES CTR RUSSIA
item PERELYGIN, V - ST RES CTR RUSSIA
item LEVCHUK, V - ST RES CTR RUSSIA
item MITSEVICH, E - ST RES CTR RUSSIA
item MITSEVICH, I - ST RES CTR RUSSIA
item BORSENKOV, V - ST RES CTR RUSSIA
item Stern, Norman

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/13/2007
Publication Date: 5/15/2007
Citation: Svetoch, E.A., Eruslanov, B.V., Perelygin, V.V., Levchuk, V.P., Mitsevich, E.V., Mitsevich, I.P., Borsenkov, V.N., Stern, N.J. 2007. Application of bacteriocin produced by Streptococcus cricetus S760 for treating Campylobacter jejuni and Salmonella-associated infections in broilers. Meeting Abstract. P. A15, Abstract #56.

Interpretive Summary:

Technical Abstract: Background: Campylobacter jejuni and Salmonella enteritidis are leading food-borne pathogens worldwide. An important natural reservoir for these pathogens is commercially distributed poultry. Objective: To control C. jejuni and S. enteritidis associated infections in broilers by treating with a purified bacteriocin (antimicrobial poly-peptide) produced by Streptococcus cricetus 760. Methods: 38 to 40 day old broilers, naturally colonized with C. jejuni (ranging from 5 ' 104 to 1.2 ' 108 CFU/g cecal material), were held in isolated containment. Salmonella infection of 2 day old chicks was accomplished by challenging per os a suspension of S. enteritidis 92 Rifr (~3 ' 107 CFU/bird). Chicks were provided drinking water containing bacteriocin S760 in the following concentrations: 3.12, 6.25, 12.5 and 25 mg/L. After treatment, birds were sacrificed to determine colonization levels of C. jejuni and S. enteritidis in cecal materials. Results: Bacteriocin S760 provided via drinking water for 1, 2 and 3 days at concentrations of 3.1, 6.2 and 12.5 mg/L, respectively, reduced both Campylobacter spp. and S. enteritidis to non-detectable levels in the ceca among more than 90% of the treated birds. S. enteritidis was not detected in the spleen and liver in 50% of treated birds. Conclusion: Bacteriocin S760, the metabolic byproduct of viable Streptococcus cricetus S760, can be used to treat and eliminate Campylobacter spp. and Salmonella enteritidis from broilers prior to slaughter.