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Title: Isolation, characterization and diverse pathogen killing by a bacteriocin from Enterococcus faecium (E 50-52)

Author
item Stern, Norman
item SVETOCH, E - ST RES CTR RUSSIA
item ERUSLANOV, B - ST RES CTR RUSSIA
item PERELYGIN, V - ST RES CTR RUSSIA
item MITSEVICH, E - ST RES CTR RUSSIA
item MITSEVICH, I - ST RES CTR RUSSIA
item BORZENKOV, V - ST RES CTR RUSSIA
item LEVCHUK, V - ST RES CTR RUSSIA
item SVETOCH, O - ST RES CTR RUSSIA

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/13/2007
Publication Date: 5/15/2007
Citation: Stern, N.J., Svetoch, E.A., Eruslanov, B.V., Perelygin, V.V., Mitsevich, E.V., Mitsevich, I.P., Borzenkov, V.N., Levchuk, V.P., Svetoch, O.E. 2007. Isolation, characterization and diverse pathogen killing by a bacteriocin from Enterococcus faecium (E 50-52). Meeting Abstract. P. A14, Abstract #51.

Interpretive Summary:

Technical Abstract: Background: Effectiveness of antibiotics to resolve bacterial disease has been reduced with the increased prevalence and magnitude of antibiotic resistance. Methods: Poultry intestinal materials were streaked onto MRS agar and incubated at 37oC for 24 and 48 h. An inhibitory zone surrounding plated Campylobacter jejuni NCTC 11168 was used for selection. The antagonist was grown in broth, the cell-free supernatant precipitated, the precipitate was dialyzed and, chromatography purified the peptide. MICs of the peptide were determined against 24 isolates of Yersinia enterocolitica, 29 Staphylococcus aureus, and 16 Listeria spp. SDS-PAGE, IEF, MALDI-TOF and amino acid sequence analyses characterized the peptide. Results: A Gram-positive cocci, grew in clusters in the presence of 6.5% NaCl and 40% bile at temperatures between 10 and 45oC, at pH 9.6 and was catalase negative. Biochemical analyses classified the organism as Enterococcus faecium E50-52 which was antagonistic to C. jejuni. The protein was tested against 31 strains of pathogens (Salmonella, E. coli, Shigella, Yersinia, Citrobacter, Klebsiella, Shigella, Staphyloccus, Pseudomonas, Proteus, Morganella and Listeria) with MIC (µg/ml) ranging from 0.1 to 1.6; against 24-Y. enterocolitica isolates MICs were 0.156 to 1.25; against 29-S. aureus isolates from 0.2 to 0.8 and; against 16-Listeria spp. isolates from 0.98 to 31.25. Bactericidal activity proven as caused by this bacteriocin. The consensus amino acid sequence was consistent with other bacteriocins. Conclusions: Bacteriocin from E. faecium E50-52 has a wide range of activity against pathogens and serves as a valuable alternative to imprudent use of antibiotics.