AFLATOXIN CONTROL THROUGH TARGETING MECHANISMS GOVERNING AFLATOXIN BIOSYNTHESIS IN CORN AND COTTONSEED
Location: Food and Feed Safety Research
Title: Development and Evaluation of an Affymetrix array for Aspergillus flavus
| Dolezal, Andrea - NCSU RALEIGH |
| Georgianna, David - NCSU RALEIGH |
| Obrian, Greg - NCSU RALEIGH |
| Woloshuk, Charles - PURDUE UNIV |
| Keller, Nancy - UNIV OF WI, MADISON |
| Nielsen, Dahlia - NCSU RALEIGH |
| Payne, Gary - NCSU RALEIGH |
Submitted to: Fungal Genetics Conference/Asilomar
Publication Type: Abstract Only
Publication Acceptance Date: February 16, 2007
Publication Date: March 18, 2007
Citation: Dolezal, A., Georgianna, D.R., Obrian, G., Woloshuk, C., Keller, N., Yu, J., Nielsen, D., Payne, G. 2007. Development and Evaluation of an Affymetrix array for Aspergillus flavus. In: Proceedings of the 24th Fungal Genetics Conference and the 4th International Aspergillus Meeting, March 18-25, 2007, Asilomar, CA. Abstract #143, p. 74.
A multi-species Affymetrix GeneChip array was developed to study development, metabolism and pathogenicity of A. flavus. This chip based on the whole genome sequence of A. flavus, contains 13,000 A. flavus genes, 8,000 maize genes and 25 human and mouse innate immune response genes, as well as the fumonisin and trichothecene clusters from Fusarium. These arrays were used to monitor gene expression of A. flavus during aflatoxin biosynthesis in defined media and during infection of developing maize seeds. A parallel study comparing this array with a 5002 element cDNA array showed the same expression profile for the aflatoxin biosynthetic genes when A. flavus was grown in culture on defined media at conducive and non-conducive temperatures for aflatoxin production. Gene expression was also monitored in A. flavus during the infection of field grown maize seeds. The profile of aflatoxin gene expression by A. flavus in infected maize kernels was similar to that observed for A. flavus grown in the lab under conductive temperatures for aflatoxin production. In addition, several genes encoding enzymes for the metabolism of complex carbohydrates and for transporters were also elevated during infection of maize seeds. In none of the experiments with the Affymetrix GeneChip arrays did we observe species cross hybridization. These initial observations show that these multi-species arrays will be a powerful tool for studying the complex ecology and metabolism of A. flavus.