ARS | Publication request: Differential effects of clathrin and actin inhibitors on internalization of Escherichia coli and Salmonella choleraesuis in porcine jejunal Peyer's patches

Submitted to: Veterinary Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: October 12, 2005
Publication Date: March 31, 2006
Citation: Green, B.T., Brown, D.R. 2006. Differential effects of clathrin and actin inhibitors on internalization of Escherichia coli and Salmonella choleraesuis in porcine jejunal Peyer's patches. Veterinary Microbiology, 113:117-122.

Interpretive Summary: This study examined the internalization of nonpathogenic and pathogenic bacteria into porcine intestinal Peyer’s patches. Peyer’s patches are the inductive site for mucosal immunity and are therefore a target for mucosal vaccines. This study provides evidence that pathogens use a different mechanism of internalization into host cells as compared to nonpathogens. Understanding the differences in internalization between pathogens and nonpathogens will aid in the development of more effective mucosal vaccines and provide a greater understanding of bacterial pathogenesis.

Technical Abstract: Peyer’s patches constitute both an inductive immune site and an enteropathogen invasion route. Peyer’s patch mucosae from porcine jejunum were mounted in Ussing chambers, and either Salmonella choleraesuis vaccine strain SC-54 or non-pathogenic rodent and porcine Escherichia coli strains contacted the Peyer’s patch mucosa for 90 min. Internalized bacteria were quantified by a gentamicin resistance assay. Monodansylcadaverine (300 'M, luminal addition), an inhibitor of clathrin-mediated endocytosis, significantly inhibited internalization of both E. coli strains relative to tissues untreated with the inhibitor; internalization of SC-54 was unaffected. The actin-disrupting agent cytochalasin D (10 'M. luminal addition), inhibited internalization of pig-adapted E. coli but not that of rodent-adapted E. coli or SC-54. Internalization of SC-54 and non pathogenic E. coli in Peyer’s patches appears to occur through different cellular routes.