Location: Livestock Issues Research
Title: Microarray analysis of norepinephrine grown E. coli O157:H7 Author
Submitted to: American Society for Microbiology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: December 15, 2006
Publication Date: May 21, 2007
Citation: Dowd, S.E. 2007. Microarray analysis of norepinephrine grown E. coli O157:H7 [abstract]. American Society for Microbiology 107th General Meeting, May 21-25, 2007, Toronto, Canada. Technical Abstract: To examine differences in the transcription profile of Escherichia coli O157:H7 during growth in the presence of catecholamines, the clinical-type isolate EDL933 (ATCC 43895) was grown using an in-vitro culture system made up of moderate initial inoculum and a serum-SAPI based medium. After 5 hrs of exposure to NE, treatment cultures and control cultures (not exposed to NE) were harvested and their RNA purified and utilized for microarray analyses. Results indicated that there was a dramatic increase in the expression of virulence factors as well as transcripts for genes involved in iron binding and iron transport. Conversely, there were comparative repressions of several iron scavenging, flagella biosynthesis, and phage shock protein-related transcripts in the presence of NE. The results corroborate many of the previous findings detailed in the literature. Expression profiles obtained could elucidate additional virulence gene induction mechanisms and promote better understanding of the genetic control of iron acquisition during growth in the presence of catecholamines.