Submitted to: American Chemical Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: November 13, 2006
Publication Date: March 29, 2007
Citation: Luthria, D.L. 2007. Influence of Sample Preparation on Assay of Functional Phenolic Phytochemicals. American Chemical Society, 233rd National Meeting & Exposition, March 25-29, 2007, Chicago, Illinois. Technical Abstract: Phenolics are one of the most diverse groups of phytochemicals that are ubiquitously distributed throughout the plant kingdom. Phenolic phytochemicals are known to exhibit a wide range of health protective effects. Approximately 8000 different phenolics compounds have been isolated from natural resources. Structural diversity, solubility, and interaction with the matrix impose a significant challenge in extraction and analysis of phenolic phytochemicals in foods and dietary supplements. This presentation will summarize our previous and current research results on the influence of extraction techniques and conditions on assay of phenolic compounds. Comparison of current (pressurized liquid extraction and ultrasonic assisted extraction) and classical extraction approaches (Soxhlet, stirring, vortexing, wrist, and rotary shakers) on assay of phenolic phytochemicals from different matrices will be presented. Influence of delipidation, extraction temperature, time, number of cycles, solid-to-solvent ratio, and pressure on assay of phenolic compounds will also be presented. This presentation will conclude with a systematic outline for optimizing procedures for extraction of phytochemicals from different food matrices. Accurate quantitation of phytochemicals will allow researchers to accurately determine the dietary intake levels and safety guidelines for potentially bioactive compounds necessary to achieve the desired health-beneficial effects. Supported by the ARS/USDA, and Dionex, Inc., USA.