|Nielsen, Niels - NORTH CAROLINA STATE UNIV|
Submitted to: Journal of Science, Agriculture and Environment
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 5, 2006
Publication Date: N/A
Interpretive Summary: The wealth of knowledge derived from sequencing the DNA from various plants can now be used to determine the function of genes. One strategy for doing this is called TILLInG, and it involves combining traditional chemical mutation of plants with modern molecular biology techniques. This article reviews the current status of TILLInG and how it is being used to develop better plant varieties to support the end users’ needs, whether they be producers, consumers or industry.
Technical Abstract: TILLInG (Targeting Induced Local Lesions In Genomes) is a reverse genetic strategy which combines traditional mutagenesis with high-throughput molecular biology protocols. TILLInG was first established in Arabidopsis thaliana when other reverse genetic strategies did not produce acceptable results. TILLInG libraries have been established in several species and more are in progress. Time must be invested in creating a good TILLInG population, such that the mutation frequency is balanced with lethality. In addition, the DNA must be stable. The number of examples of the usefulness of TILLInG libraries is continually growing. While there are some disadvantages, most can be overcome. The flexibility of TILLInG has established it as a useful strategy with great potential to advance agriculture.