|Tai, Yin Shan|
|Bragg, Jennifer - UC BERKELEY|
Submitted to: Biotechnology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 3, 2006
Publication Date: June 16, 2007
Citation: Tai, Y., Bragg, J. 2007. Dual applications of a virus vector for studies of wheat-fungal interactions. Biotechnology 6 (2):288-291. Interpretive Summary: In the post-genomic era, research on functional genomics is a major trend. One essential feature needed for much of this work is the ability to investigate gene function in a high-throughput manner. To verify the function of a given gene, stable transgenic plants are often generated as a routine approach for most plant species. However, obtaining transgenic wheat plants for this purpose is still a highly challenging barrier because of the nature of wheat and technical limitations. This work presents an alternative method to study gene function in wheat. To help understand a gene’s function, one can either decrease (gene silencing) or increase (overexpression) the level of gene expression. We previously developed and reported a virus vector that could be used as a tool for gene silencing in wheat in moderately high-throughput applications. Now we extend the utilization of this virus vector system into an expression system. To demonstrate its applicability, we have applied it to the study of ToxA, a fungal toxin important in tan spot disease of wheat. We further discuss the possible application of this system to fungal functional genomics.
Technical Abstract: Obtaining stable transgenic plants is still a highly challenging barrier for analyses of gene function in wheat. Unlike the situation with dicots and other model organisms, RNA silencing and transient assays of gene expression are not well-established in wheat. We previously developed and reported a virus-induced gene silencing (VIGS) system in wheat, and now we present here a transient expression system for assays in wheat. To demonstrate the utility of this transient assay system, we have applied it to the study of ToxA, a fungal toxin important in tan spot disease of wheat.